Life Sciences Commons^™

Exploration Of Student Biodiversity Knowledge And Decision-Making For A Wildlife Conservation Socioscientific Issue, Ashley R. Alred

School of Natural Resources: Dissertations, Theses, and Student Research

Global biodiversity, a foundation for ecosystem function, is diminishing at a rate unprecedented in the last 50 years. Biodiversity loss and ecosystem services deterioration is linked to increased food insecurity, reduced water quality and availability, decreased energy security, higher economic losses and human suffering (Millennium Ecosystem Assessment, 2005). Consequently, educators should invest in supporting students in their development of ecological understanding and formal decision-making skills so they are equipped with meaningful tools they can use as scientifically literate citizens. To contribute to that mission, this study seeks to explore student 1) comprehension and explanation of biodiversity concepts and 2) decision-making …

Characterization Of Molecular Communication Based On Cell Metabolism Through Mutual Information And Flux Balance Analysis, Zahmeeth Sayed Sakkaff

Department of Computer Science and Engineering: Dissertations, Theses, and Student Research

Synthetic biology is providing novel tools to engineer cells and access the basis of their molecular information processing, including their communication channels based on chemical reactions and molecule exchange. Molecular communication is a discipline in communication engineering that studies these types of communications and ways to exploit them for novel purposes, such as the development of ubiquitous and heterogeneous communication networks to interconnect biological cells with nano and biotechnology-enabled devices, i.e., the Internet of Bio-Nano Things. One major problem in realizing these goals stands in the development of reliable techniques to control the engineered cells and their behavior from the …

Investigation Of Lipid Profile Patterns Along Latitude In An Eastern Australian Cline And After Stress Selection In The Laboratory, And Experiments On Stress Tolerance With Rnai Knockdown Of Lipid Biosynthesis Genes, Li Ko

School of Biological Sciences: Dissertations, Theses, and Student Research

Homeoviscous adaptation in membranes and changes in energy storage lipids such as triacylglycerols (TAGs) are the two main themes of adaptation to environmental conditions. Basal patterns of lipid compositional profile of Drosophila melanogaster along a latitudinal gradient in the eastern coast of Australia indicate variations of lipidome associated with climatic patterns. Lipidome data were collected for eight locations along the eastern Australian cline for both sexes in D. melanogaster and D. simulans. To further investigate the relationship between lipidome patterns and environmental factors, we utilized laboratory selection studies using stress conditions including high temperature tolerance, desiccation survival, and chill-coma …

The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin

School of Biological Sciences: Dissertations, Theses, and Student Research

Due to the increased number of immunocompromised patients, infections by Candida albicans have significantly increased in recent years. C. albicans transition from yeast to germ tubes is an essential factor for virulence. In this study we noted that Lee's medium, commonly used to induce filamentation, contained 500-fold more biotin than needed for growth. Thus, we investigated the effects of excess biotin on growth rate and filamentation by C. albicans in different media. At 37 °C, excess biotin (4 µM) enhanced germ tube formation (GTF) ca. 10-fold in both Lee's medium and a defined glucose proline medium, and ca. 4-fold in …

Inter- And Intra-Individual Variation In Predator-Related Behavioral Plasticity Expressed By Female Green Swordtails (Xiphophorus Hellerii), Rachael A. Disciullo

School of Biological Sciences: Dissertations, Theses, and Student Research

Phenotypic plasticity is the ability of one genotype to express multiple phenotypes under variable environments. Behavioral plasticity is a type of phenotypic plasticity in which individuals adjust behavior in response to changes in environment. Often, behavioral plasticity is studied at the level of the population, rather than at the level of the individual. Further, few studies have considered the effect of individual traits, such as size and age, on the expression of behavioral plasticity, or, how individual plasticity may be correlated across different contexts. In this study, we used female green swordtails (Xiphophorus hellerii) to test the effects …

Polyandry In The Absence Of Fitness Benefits In A Species With Female-Biased Sexual Size Dimorphism, Marie Claire Chelini, Eileen A. Hebets

Eileen Hebets Publications

Most studies exploring the evolution of female mating systems focus on species in which females are either monandric (mate with a single male) or highly polyandric (mate with multiple males), but less is understood about variation in mating decisions within a species. How and why do females of a single species decide whether or not to copulate with additional mates? In this study we attempt to answer this question in the highly dimorphic crab spider, Mecaphesa celer, whose females may be either monandric or polyandric. We tested three hypotheses: (1) a female’s decision to remate is based on sequential mate …

Characterization Of The Role Of Host And Dietary Factors In The Establishment Of Bacteria In The Gastrointestinal Tract, Janina A. Krumbeck

School of Biological Sciences: Dissertations, Theses, and Student Research

Probiotic bacteria and synbiotics are used as therapeutic and prophylactic agents. The majority of probiotic and synbiotic applications contain bacterial strains that are allochthonous to the human gastrointestinal (GI) tract. Accordingly, many bacterial strains do not survive digestion, or are not capable of persisting and competing the resident gut microbiota, and are therefore washed out of the GI tract shortly after the treatment is discontinued. This might reduce the health effects of these treatments. Therefore, research is needed to address the ecological challenges that probiotic strains encounter in the GI tract in order to develop probiotic regimens. Determining which ecological …

Sex Chromosome-Wide Transcriptional Suppression And Compensatory Cis-Regulatory Evolution Mediate Gene Expression In The Drosophila Male Germline, Emily L. Landeen, Christina A. Muirhead, Lori Wright, Colin D. Meiklejohn, Daven C. Presgraves

School of Biological Sciences: Faculty Publications

The evolution of heteromorphic sex chromosomes has repeatedly resulted in the evolution of sex chromosome-specific forms of regulation, including sex chromosome dosage compensation in the soma and meiotic sex chromosome inactivation in the germline. In the male germline of Drosophila melanogaster, a novel but poorly understood form of sex chromosome-specific transcriptional regulation occurs that is distinct from canonical sex chromosome dosage compensation or meiotic inactivation. Previous work shows that expression of reporter genes driven by testis-specific promoters is considerably lower—approximately 3-fold or more—for transgenes inserted into X chromosome versus autosome locations. Here we characterize this transcriptional suppression of X-linked …

The Maintenance Of Phenotypic Divergence Through Sexual Selection: An Experimental Study In Barn Swallows Hirundo Rustica, Rebecca Safran, Yoni Vortman, Brittany R. Jenkins, Joanna K. Hubbard, Matt Wilkins, Rachel J. Bradley, Arnon Lotem

School of Biological Sciences: Faculty Publications

Previous studies have shown that sexual signals can rapidly diverge among closely related species. However, we lack experimental studies to demonstrate that differences in trait-associated reproductive performance maintain sexual trait differences between closely related populations, in support for a role of sexual selection in speciation. Populations of Northern Hemisphere distributed barn swallows Hirundo rustica are closely related, yet differ in two plumage-based traits: ventral color and length of the outermost tail feathers (streamers). Here we provide experimental evidence that manipulations of these traits result in different reproductive consequences in two subspecies of barn swallow: (H. r. erythrogaster in North …

Range-Wide Patterns Of Geographic Variation In Songs Of Golden-Crowned Sparrows (Zonotrichia Atricapilla), Daizaburo Shizuka, M Ross Lein, Glen Chilton

School of Biological Sciences: Faculty Publications

Discrete geographic variation, or dialects, in songs of songbirds arise as a consequence of complex interactions between ecology and song learning. Four of the five species of Zonotrichia sparrows, including the model species White-crowned Sparrow (Z. leucophrys), have been studied with respect to the causes and consequences of geographic variation in song. Within White-crowned Sparrows, subspecies that migrate farther have larger range size of dialects. Here, we assessed geographic patterns of song variation in the fifth species of this genus, the Golden-crowned Sparrow (Z. atricapilla). We analyzed field-recorded songs from 2 sampling periods (1996–1998 and 2006–2013) …

Genome-Wide Differentiation In Closely Related Populations: The Roles Of Selection And Geographic Isolation, Rebecca Safran, Elizabeth Scordato, Matt Wilkins, Joanna K. Hubbard, B. R. Jenkins, Tomáš Albrecht, Samuel Flaxman, H. Karaardiç, Yoni Vortman, Arnon Lotem, Patrik Nosil, P. Pap, Sheng-Feng Shen, S.-F. Chan, T. L. Parchman, Nolan C. Kane

School of Biological Sciences: Faculty Publications

Population divergence in geographic isolation is due to a combination of factors. Natural and sexual selection may be important in shaping patterns of population differentiation, a pattern referred to as “isolation by adaptation” (IBA). IBA can be complementary to the well-known pattern of “isolation by distance” (IBD), in which the divergence of closely related populations (via any evolutionary process) is associated with geographic isolation. The barn swallow Hirundo rustica complex comprises six closely related subspecies, where divergent sexual selection is associated with phenotypic differentiation among allopatric populations. To investigate the relative contributions of selection and geographic distance to genome-wide differentiation, …

Nocturnal Foraging Enhanced By Enlarged Secondary Eyes In A Net-Casting Spider, Jay A. Stafstrom, Eileen A. Hebets

Eileen Hebets Publications

Animals that possess extreme sensory structures are predicted to have a related extreme behavioral function. This study focuses on one such extreme sensory structure—the posterior median eyes of the net-casting spider Deinopis spinosa. Although past research has implicated the importance of vision in the nocturnal foraging habits of Deinopis, no direct link between vision in the enlarged eyes and nocturnal foraging has yet been made. To directly test the hypothesis that the enlarged posterior median eyes facilitate visually based nocturnal prey capture, we conducted repeated-measures, visual occlusion trials in both natural and laboratory settings. Our results indicate that D. …

Depression In Thermal Performance Of Age-Structured Spirodela Polyrhiza Due To The Presence Of Rhopalosiphum Nymphaeae, Mitchell Matis, Chad Brassil, Colby J. Tanner

UCARE Research Products

Thermal performance curves are keys components of population ecology. We performed this study to determine the effects of aphids on duckweed age-structured models across temperature. Results show a depression in birth rates and maturation of rates of duckweed in the presence of aphids. Regression analysis shows that this depression in maturation and birth is directly proportional to aphid growth rates across temperatures. This hints of the idea of modeling duckweed and aphid growth via nested thermal performance curves.

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network [Special Feature: Neon Design], Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott Lyell Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Laura D. Kramer, Amy J. Kuenzi, Shannon L. Ladeau, Todd P. Livdahl, James N. Mills, Chester G. Moore, Serge Morand, Roger S. Nasci, Nicholas H. Ogden, Richard S. Ostfeld, Robert R. Parmenter, Joseph Piesman, William K. Reisen, Harry M. Savage, Daniel E. Sonenshine, Andrea Swet, Michael J. Yabsley

Scott L. Gardner Publications

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Population And Morphological Changes In American Kestrels Through Space And Time, Teresa E. Ely

School of Biological Sciences: Dissertations, Theses, and Student Research

A once common raptor, the American kestrel (Falco sparverius) has experienced population declines in the last two decades throughout North America. Many hypotheses exist about the decline, including mortality from West Nile virus, rodenticide poisoning, climate change, an increase in predators, and core habitat loss or degradation, which could influence food availability. Food availability is key to raptor survival and reproduction, and changes in food availability throughout the year can have lifelong effects on size and body condition. Here we examine how morphology, specifically mass and wing chord, has changed at seven migration sites throughout North America as …

Factors Affecting The Immune System Of The Ornate Box Turtle (Terrapene Ornata), Michelle W. Mcquinn, Abigail A. Neyer, Gwendolyn C. Bachman

UCARE Research Products

While many studies have detailed the complex intricacies of the endothermic immune system, relatively little is known about the immune system of ectotherms--specifically, reptiles. In an attempt to gain more knowledge about the factors affecting reptilian immune function, ornate box turtles (Terrapene ornata) were subjected to chronic stress in the form of high ambient temperatures in a semi-natural environment. It was hypothesized that chronic stress would lead to elevated levels of corticosterone in the blood, which would, in turn, suppress immune activity. It was found that body temperature and body mass in particular were significantly affected by chronic heat stress. …

Assessing Student Comprehension In Introductory Biology: A Comparison Of Free-Response And Multiple-True/False Exam Formats, Macy A. Potts, Brian Couch, Joanna K. Hubbard

UCARE Research Products

Question format is an important consideration when designing instruments to gauge student comprehension. In many lecture-style courses, instructors must decide how to effectively test a large group of students. Two common types of question formats used for introductory biology exams are free-response (FR) and multiple-true/false (MTF). FR questions include a question prompt which students respond to with essay-style answers. Conversely, MTF questions include an opening question stem with accompanying statements to be marked as either true or false. The goal of this study was to determine the advantages and disadvantages of FR and MTF question formats for assessing student comprehension …

No Nonsense: The Protection Of Wild-Type Mrnas From Nonsense-Mediated Mrna Decay In Saccharomyces Cerevisiae, Krista Patefield

School of Biological Sciences: Dissertations, Theses, and Student Research

Gene regulation in eukaryotes is tightly controlled at multiple levels to ensure proper expression and cellular homeostasis. Misregulation of gene expression is a common source of genetic disease. One mechanism by which cells are able to control gene expression is through the synthesis and degradation of the mRNA molecules encoding the genes. The transcription and degradation of mRNA molecules controls the pool mRNAs that are available to the translational machinery. One of the well-studied mRNA decay pathways is the Nonsense-Mediated mRNA Decay pathway (NMD). Originally, NMD was discovered as a posttranscriptional mRNA surveillance mechanism responsible for the deadenylation-independent decapping and …

Molecular Analysis Confirming The Introduction Of Nile Crocodiles, Crocodylus Niloticus Laurenti 1768 (Crocodylidae), In Southern Florida, With An Assessment Of Potential For Establishment, Spread, And Impacts., Michael R. Rochford, Kenneth L. Krysko, Frank J. Mazzotti, Matthew W. Shirley, Mark W. Parry, Joseph A. Wasilewski, Jeffrey S. Beauchamp, Christpher R. Gillette, Edward F. Metzger Iii, Michiko A. Squires, Louis A. Somma

Papers in Herpetology

The state of Florida, USA, has more introduced herpetofauna than any other governmental region on Earth. Four species of nonnative crocodilians have been introduced to Florida (all since 1960), one of which is established. Between 2000–2014 we field-collected three nonnative crocodilians in Miami-Dade County, Florida, and one in Hendry County, Florida. We used DNA barcoding and molecular phylogenetics to determine species identification and native range origin. Also, we described diet, movement, and growth for one crocodile. Our molecular analyses illustrated that two of the crocodiles we collected are most closely related to Nile Crocodiles (Crocodylus niloticus) from South Africa, suggesting …

Computational Modeling Intervention: Using Dynamical Models To Teach Complex Biological Processes, Audrey J. Crowther, Tomáš Helikar, Nicholas Galt, Joseph Dauer, Heather Berganr, Bryan Kowal

UCARE Research Products

The Unites States, while being one of the richest countries in the world, ranks 17th in science proficiency out of 31 countries. New techniques for teaching are needed if the US wants to keep up with increasing global competition. Often the problem with traditional methods for teaching biological processes is that they present the material as linear or causal, when in fact there is a much wider network at play. For example, cellular respiration is often represented as a linear process that always starts with glucose and always ends with ATP and water. However, there are several entry and exit …

What Are The Genes That Cause Male Sterility In Hybrid Offspring Between Drosophila Mauritiana And Drosophila Simulans?, Naznaz J. Majid, Colin D. Meiklejohn

UCARE Research Products

The purpose of this research project is to better understand speciation -- the process by which new species arise. To better understand how species form we will investigate how reproductive barriers come about-specifically the sterility of hybrid offspring will be the focus of this research. The species under investigation are Drosophila mauritiana and D. simulans that produce sterile hybrid males. These species are used specifically because they produce fertile hybrid females which allow genes to be moved between them. There are also published genome sequences for both species, which makes it is easier to identify candidate genes. These species are …

Amp Expression In Energetic Hybrid D. Melanogaster Infected With P. Rettgeri, Cassandra K. Treu, Justin Buchanan, Kristi Montooth

UCARE Research Products

The purpose of this experiment was to investigate immune function and energy metabolism, particularly the levels of antimicrobial peptides produced in Drosophila energetically compromised genotypes. This will provide the ability to investigate energetics of immunity without changing diet. Flies were infected with the bacteria P. rettgeri and the resulting immune response was investigated. Females did not mount as effective an immune response as males. All flies exhibited decreased survivorship from infection. Control flies survived at higher levels and showed no mitochondrial:nuclear interaction.

Identification And Characterization Of Ion Channel Activity Of The M2 Protein From Influenza Virus D (Dm2), Jianing Liu

School of Biological Sciences: Dissertations, Theses, and Student Research

Viral ion channels are membrane proteins of influenza viruses that play essential roles in the replication cycle, which enables them to be targeted by antiviral drugs. M2 of influenza type A virus, BM2 of influenza type B virus, and CM2 from influenza type C virus have been characterized as ion channel proteins and antiviral drug amantadine was developed to control influenza type A virus. However, few studies have been conducted to clarify the properties of the M2 protein (DM2) of influenza type D virus, a novel influenza virus genus identified in 2014. To identify the ion channel activity of DM2, …

Brain Tumor In A Dish: Glioma/Astrocyte Co-Cultures As A Model For In Vitro Studies, Erin Eickman, Christina Wilson, Srivatsan Kidambi

UCARE Research Products

This study seeks to engineer an in vitro co-culture model to elucidate the role of glioma-astrocyte interactions on molecular changes in the tumor microenvironment. The use of patterned co-cultures created with polyelectrolyte multilayers and micromolding in capillaries will allow tthe investigation of cell-cell communication. This study will lead to better understanding of the role of healthy cells in cancer progression and potential treatment options.

Molecular Evolution And Functional Divergence Of Trace Amine–Associated Receptors, Seong-Il Eyun, Hideaki Moriyama, Federico G. Hoffmann, Etsuko N. Moriyama

School of Biological Sciences: Faculty Publications

Trace amine-associated receptors (TAARs) are a member of the G-protein-coupled receptor superfamily and are known to be expressed in olfactory sensory neurons. A limited number of molecular evolutionary studies have been done for TAARs so far. To elucidate how lineage-specific evolution contributed to their functional divergence, we examined 30 metazoan genomes. In total, 493 TAAR gene candidates (including 84 pseudogenes) were identified from 26 vertebrate genomes. TAARs were not identified from non-vertebrate genomes. An ancestral-type TAAR-like gene appeared to have emerged in lamprey.We found four therian-specific TAAR subfamilies (one eutherian-specific and three metatherian- specific) in addition to previously known nine …

Comparative Population Genomics Of Latitudinal Variation In Drosophila Simulans And Drosophila Melanogaster, Heather E. Machado, Alan O. Bergland, Katherine R. O'Brien, Emily L. Behrman, Paul S. Schmidt, Dmitri A. Petrov

School of Biological Sciences: Faculty Publications

Examples of clinal variation in phenotypes and genotypes across latitudinal transects have served as important models for understanding how spatially varying selection and demographic forces shape variation within species. Here, we examine the selective and demographic contributions to latitudinal variation through the largest comparative genomic study to date of Drosophila simulans and Drosophila melanogaster, with genomic sequence data from 382 individual fruit flies, collected across a spatial transect of 19 degrees latitude and at multiple time points over 2 years. Consistent with phenotypic studies, we find less clinal variation in D. simulans than D. melanogaster, particularly for the autosomes. Moreover, …

Msh1 Is A Plant Organellar Dna Binding And Thylakoid Protein Under Precise Spatial Regulation To Alter Development, Kamaldeep S. Virdi, Yashitola Wamboldt, Hardik Kundariya, John D. Laurie, Ido Keren, K.R. Sunil Kumar, Anna Block, Gilles J. Basset, Steve Luebker, Christian Elowsky, Philip M. Day, Johnna L. Roose, Terry M. Bricker, Thomas Elthon, Sally A. Mackenzie

School of Biological Sciences: Faculty Publications

As metabolic centers, plant organelles participate in maintenance, defense, and signaling. MSH1 is a plantspecific protein involved in organellar genome stability in mitochondria and plastids. Plastid depletion of MSH1 causes heritable, non-genetic changes in development and DNA methylation. We investigated the msh1 phenotype using hemi-complementation mutants and transgene-null segregants from RNAi suppression lines to sub-compartmentalize MSH1 effects. We show that MSH1 expression is spatially regulated, specifically localizing to plastids within the epidermis and vascular parenchyma. The protein binds DNA and localizes to plastid and mitochondrial nucleoids, but fractionation and protein–protein interactions data indicate that MSH1 also associates with the thylakoid …

A Stenotrophomonas Maltophilia Strain Evades A Major Caenorhabditis Elegans Defense Pathway, Corin V. White, Brian J. Darby, Robert J. Breeden, Robert J. Breeden, Michael A. Herman

School of Biological Sciences: Faculty Publications

Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic pathways and pathway components with higher organisms, the study of its interaction with bacterial pathogens has biomedical implications. S. maltophilia has been isolated in association with nematodes from grassland soils, and it is likely that C. elegans encounters this bacterium in nature. We found that a local S. maltophilia isolate, JCMS, is more virulent than the other S. maltophilia …

"My Paintings Would Be No Different Than A Picture In A Biology Textbook", Andi Kur

UReCA: The NCHC Journal of Undergraduate Research & Creative Activity

I find that there are innate balances in life, universal dichotomies that permeate our understanding of the world. My paintings are about a duality such as this that exists between art and science. We are told from youth that these subjects are poles in constant strain, as miscible as oil in water. I spent thirteen years in school believing that I must choose between the two, that it is unnecessary to carry both with me. Drawn between a distinct love of each, I realized how vehemently I disagreed. Everything: every rock to every tree to every person is suspended between …

A Dataset For Assessing Temporal Changes In Gene Expression During The Aging Process Of Adult Drosophila Melanogaster, Kimberly A. Carlson, Chi Zhang, Lawrence G. Harshman

School of Biological Sciences: Faculty Publications

A Drosophila melanogaster genome-wide transcriptome dataset is

available for studies on temporal patterns of gene expression. Gene

expression was measured using two-dye color oligonucleotide arrays

derived from Version 2 of the Drosophila Genomics Resource Center.

A total of 15,158 oligonucleotide probes corresponded to a high

proportion of the coding genes in the genome. The source of the flies

was a highly genetically heterogeneous population maintained in an

overlapping generation population regime. This regime was

designed to maintain life history traits so that they were similar to

those found in natural populations. Flies collected for the cohorts

were obtained in a …