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A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
Turkish Journal of Biology
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.