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A Study Of The Endogenous Electron Donors For Nitrogen Fixation By Cell-Free Extracts Of Klebsiella Pneumoniae M5al (Aerobacter Aerogenes M5al), Elisabeth Yi-Jan Wu
A Study Of The Endogenous Electron Donors For Nitrogen Fixation By Cell-Free Extracts Of Klebsiella Pneumoniae M5al (Aerobacter Aerogenes M5al), Elisabeth Yi-Jan Wu
Electronic Theses and Dissertations
Biological nitrogen fixation, the mechanism of the enzymatic conversion of the inert nitrogen gas into amino acids in the cytoplasm of the microorganism has puzzled many scientists for decades. The recent discovery of some free-living nitrogen-fixing bacteria has encouraged the investigation of this mechanism. A big step taken in the rapid elucidation of this mechanism was the first reproducible cell-free nitrogen-fixing system from Clostridium pasteurianum successfully obtained by Carnahan et al. (9) in 1960. In the following decade, reproducible cell-free systems were demonstrated with the strict aerobe, Azotobacter vinelandii (4), facultative anaerobic Bacillus polymyxa (16) and Klebsiella pneumoniae (31) and …