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Full-Text Articles in Life Sciences

Development Of Real-Time Pcr Assays For Rapid Detection Of Pfiesteria Piscicida And Related Dinoflagellates, Torstein Tengs Nov 2000

Development Of Real-Time Pcr Assays For Rapid Detection Of Pfiesteria Piscicida And Related Dinoflagellates, Torstein Tengs

Dr. Torstein Tengs

Pfiesteria complex species are heterotrophic and mixotrophic dinoflagellates that have been recognized as harmful algal bloom species associated with adverse fish and human health effects along the East Coast of North America, particularly in its largest (Chesapeake Bay in Maryland) and second largest (Albermarle- Pamlico Sound in North Carolina) estuaries. In response to impacts on human health and the economy, monitoring programs to detect the organism have been implemented in affected areas. However, until recently, specific identification of the two toxic species known thus far, Pfiesteria piscicida and P. shumwayae (sp. nov.), required scanning electron microscopy (SEM). SEM is a …


Eight Ways To Be A Colonizer; Two Ways To Be An Invader., Mark Davis, K Thompson Jul 2000

Eight Ways To Be A Colonizer; Two Ways To Be An Invader., Mark Davis, K Thompson

Mark Davis

No abstract provided.


Phylogenetic Analyses Indicate That The 19'Hexanoyloxy-Fucoxanthin-Containing Dinoflagellates Have Tertiary Plastids Of Haptophyte Origin, Torstein Tengs May 2000

Phylogenetic Analyses Indicate That The 19'Hexanoyloxy-Fucoxanthin-Containing Dinoflagellates Have Tertiary Plastids Of Haptophyte Origin, Torstein Tengs

Dr. Torstein Tengs

The three anomalously pigmented dinoflagellates Gymnodinium galatheanum, Gyrodinium aureolum, and Gymnodinium breve have plastids possessing 199-hexanoyloxy-fucoxanthin as the major carotenoid rather than peridinin, which is characteristic of the majority of the dinoflagellates. Analyses of SSU rDNA from the plastid and the nuclear genome of these dinoflagellate species indicate that they have acquired their plastids via endosymbiosis of a haptophyte. The dinoflagellate plastid sequences appear to have undergone rapid sequence evolution, and there is considerable divergence between the three species. However, distance, parsimony, and maximum-likelihood phylogenetic analyses of plastid SSU rRNA gene sequences place the three species within the haptophyte clade. …


Heteroduplex Mobility Assay-Guided Sequence Discovery: Elucidation Of The Small Subunit (18s) Rdna Sequences Of Pfiesteria Piscicida And Related Dinoflagellates From Complex Algal Culture And Environmental Sample Dna Pools, Torstein Tengs Apr 2000

Heteroduplex Mobility Assay-Guided Sequence Discovery: Elucidation Of The Small Subunit (18s) Rdna Sequences Of Pfiesteria Piscicida And Related Dinoflagellates From Complex Algal Culture And Environmental Sample Dna Pools, Torstein Tengs

Dr. Torstein Tengs

The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, and with a novel clinical syndrome of impaired cognition and memory disturbance among humans after presumptive toxin exposure. As a result, there is a pressing need to better characterize the organism and these associations. Advances in Pfiesteria research have been hampered, however, by the absence of genomic sequence data. We employed a sequencing strategy directed by heteroduplex mobility assay to detect Pfiesteria piscicida 18S rDNA ‘‘signature’’ sequences in complex pools of DNA and used those data as the basis for determination …


Water Fog For Repelling Birds, Larry Clark, Thomas Nachtman, John Hull Jan 2000

Water Fog For Repelling Birds, Larry Clark, Thomas Nachtman, John Hull

Larry Clark

No abstract provided.


Size Selectivity Of Crappie Angling, Leandro E. Miranda, Brian S. Dorr Jan 2000

Size Selectivity Of Crappie Angling, Leandro E. Miranda, Brian S. Dorr

Brian S Dorr

Abstract.—Over 6,000 black crappies Pomoxis nigromaculatus and white crappies P. annularis were tagged in five lakes and reservoirs to quantify size selectivity of angling. Total length of fish tagged ranged from 20.0 to 39.8 cm; fish caught by anglers ranged from 20.0 to 38.8 cm. Return rates were low at lengths near 20 cm, increased gradually to a peak between 26 and 32 cm, and decreased for longer fish. This pattern was consistent among the five lakes and reservoirs and did not differ between species. Observed size-selective exploitation resulted in the lopsided removal of intermediate age-classes, thereby simulating a reversed …


Additive And Nonadditive Effects Of Herbivory And Competition On Tree Seedling Mortality, Growth, And Allocation, Scott J. Meiners, Steven N. Handel Jan 2000

Additive And Nonadditive Effects Of Herbivory And Competition On Tree Seedling Mortality, Growth, And Allocation, Scott J. Meiners, Steven N. Handel

Scott J. Meiners

The interaction between simulated cotyledon herbivory and interspecific competition was studied in a greenhouse experiment using two species of trees, Acer rubrum and Quercus palustris, which commonly invade abandoned agricultural fields. Herbivory treatments were applied as a gradient of cotyledon removal for A. rubrum with 0, 25, 50, 75, and 100% of cotyledon tissue removed. Cotyledons from Q. palustris were clipped and removed (control, early, and late removal) to create a gradient of seed reserve availability. The competition treatment consisted of plugs of old-field vegetation that filled the pots with perennial cover. Mortality of seedlings was higher with competition. There …


Predation Rates On Real And Artificial Nests Of Grassland Birds, William B. Davison, Eric K. Bollinger Jan 2000

Predation Rates On Real And Artificial Nests Of Grassland Birds, William B. Davison, Eric K. Bollinger

Eric K. Bollinger

We estimated nesting success at real and artificial nests of grassland birds to test the influence of nest type, nest position, and egg size on predation rates. We distributed wicker nests and realistic woven-grass nests baited with a clay egg and either a Northern Bobwhite (Colinus virginianus) egg or a House Sparrow (Passer domesticus) egg in four grasslands that were part of the Conservation Reserve Program in east-central Illinois. Nesting success averaged 86.5% for 12 days of exposure for artificial nests. For real nests, nesting success was markedly lower, averaging 39% over the entire nesting cycle and 59% during approximately …


Season And Distance From Forest - Old Field Edge Affect Seed Predation By White-Footed Mice, Scott J. Meiners, James T. Mccormick Jan 2000

Season And Distance From Forest - Old Field Edge Affect Seed Predation By White-Footed Mice, Scott J. Meiners, James T. Mccormick

Scott J. Meiners

We studied the spatial pattern of seed predation across a forest-old field edge in both fall and winter to assess the potential for seed predators to influence plant spatial patterns. We used a 100 x 100 m grid that began 30 m inside the forest and extended 60 m into the old field. Inside this grid we placed seed stations at regular 10 m intervals and monitored seed removal. Seed predation varied significantly across the edge gradient in both fall and winter with the highest rate of seed removal at the edge in both trials. The spatial pattern of seed …


Sagina (Caryophyllaceae) In Illinois: An Update, Gordon C. Tucker Jan 2000

Sagina (Caryophyllaceae) In Illinois: An Update, Gordon C. Tucker

Gordon C. Tucker

No abstract provided.