Life Sciences Commons^™

Characterization Of Chlamydomonas Reinhardtii Core Histones By Top-Down Mass Spectrometry Reveals Unique Algae-Specific Variants And Post-Translational Modifications, Aliyya Khan, Carlo Eikani, Hana Khan, Anthony Iavarone, James Pesavento

School of Science Faculty Works

The unicellular microalga Chlamydomonas reinhardtii has played an instrumental role in the development of many new fields (bioproducts, biofuels, etc.) as well as the advancement of basic science (photosynthetic apparati, flagellar function, etc.). Chlamydomonas' versatility ultimately derives from the genes encoded in its genome and the way that the expression of these genes is regulated, which is largely influenced by a family of DNA binding proteins called histones. We characterize C. reinhardtii core histones, both variants and their post-translational modifications, by chromatographic separation, followed by top-down mass spectrometry (TDMS). Because TDMS has not been previously used to study Chlamydomonas proteins, …