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Amp-Activated Protein Kinase Is Involved In Hormone-Induced Mouse Oocyte Meiotic Maturation In Vitro, Jing Chen, Stephen Downs
Amp-Activated Protein Kinase Is Involved In Hormone-Induced Mouse Oocyte Meiotic Maturation In Vitro, Jing Chen, Stephen Downs
Biological Sciences Faculty Research and Publications
We have previously shown that AMP-activated protein kinase (AMPK) can induce the resumption of meiosis in mouse oocytes maintained in meiotic arrest in vitro. The present study was carried out to determine whether AMPK activation is involved in hormone-induced maturation. Follicle-stimulating hormone (FSH) and the EGF-like peptide, amphiregulin (AR), are potent inducers of maturation in cumulus cell-enclosed oocytes (CEO). Within 3 h of FSH treatment, phospho-acetyl CoA carboxylase (ACC) levels were increased in germinal vesicle (GV)-stage oocytes when compared to non-stimulated controls and remained elevated throughout 9 h of culture, indicating AMPK activation. A similar response to AR was observed …