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Full-Text Articles in Life Sciences
Fiber Number And Type Composition In Extensor Digitorum Longus, Soleus, And Diaphragm Muscles With Aging In Fisher 344 Rats, Thomas J. Eddinger, Richard L. Moss, Robert G. Cassens
Fiber Number And Type Composition In Extensor Digitorum Longus, Soleus, And Diaphragm Muscles With Aging In Fisher 344 Rats, Thomas J. Eddinger, Richard L. Moss, Robert G. Cassens
Biological Sciences Faculty Research and Publications
Histochemical (M-ATPase) fiber typing was done on extensor digitorum longus, (EDL), soleus (SOL), and diaphragm (DIA) muscles of barrier-reared Fisher 344 rats obtained at four different ages (3, 9, 28, and 30 months) from the colonies of the National Institute of Aging. In the EDL there are no differences in the percent of type I fibers among the four age groups. The percent of type IIa and IIb fibers also showed no difference between the 3 and 30 month age groups. There was no apparent trend for an increase or decrease in the percent of type IIa or IIb fibers …
Nodule Initiation Elicited By Noninfective Mutants Of Rhizobium Phaseoli, Kathryn A. Vandenbosch, K. Dale Noel, Yasuko Kaneko, Eldon H. Newcomb
Nodule Initiation Elicited By Noninfective Mutants Of Rhizobium Phaseoli, Kathryn A. Vandenbosch, K. Dale Noel, Yasuko Kaneko, Eldon H. Newcomb
Biological Sciences Faculty Research and Publications
Rhizobium phaseoli CE106, CE110, and CE115, originally derived by transposon mutagenesis (Noel et al., J. Bacteriol. 158:149-155, 1984), induced the formation of uninfected root nodule-like swellings on bean (Phaseolus vulgaris). Bacteria densely colonized the root surface, and root hair curling and initiation of root cortical-cell divisions occurred normally in mutant-inoculated seedlings, although no infection threads formed. The nodules were ineffective, lacked leghemoglobin, and were anatomically distinct from normal nodules. Ultrastructural specialization for ureide synthesis, characteristic of legumes that form determinate nodules, was absent. Colony morphology of the mutant strains on agar plates was less mucoid than that of …
Dna Synthesis, Methylation And Degradation During Conjugation In Tetrahymena Thermophila, Gail Singer Harrison, Kathleen M. Karrer
Dna Synthesis, Methylation And Degradation During Conjugation In Tetrahymena Thermophila, Gail Singer Harrison, Kathleen M. Karrer
Biological Sciences Faculty Research and Publications
We have investigated the timing of DNA synthesis, methylation and degradation during macronuclear development in the ciliate, Tetrahymena thermophila. DNA synthesis was first detected in the anlagen early in macronuclear development, but the majority of DNA synthesis occurred later, after pair separation. Anlagen DNA was first detectably methylated at GATC sites 3–5 hours after its synthesis. Once initiated, de novo methylation was rapid and complete, occurring between 13.5 and 15 hours of conjugation. The level of methylation of GATC sites was constant throughout the remainder of conjugation, and was similar to that in mock-conjugated cells. Degradation of DNA in …