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Isolation And Biophysical Study Of Fruit Cuticles, Subhasish Chatterjee, Sayantani Sarkar, Julia Oktawiec, Zhantong Mao, Olivia Niitsoo, Ruth E. Stark
Isolation And Biophysical Study Of Fruit Cuticles, Subhasish Chatterjee, Sayantani Sarkar, Julia Oktawiec, Zhantong Mao, Olivia Niitsoo, Ruth E. Stark
Publications and Research
The cuticle, a hydrophobic protective layer on the aerial parts of terrestrial plants, functions as a versatile defensive barrier to various biotic and abiotic stresses and also regulates water flow from the external environment.1 A biopolyester (cutin) and long-chain fatty acids (waxes) form the principal structural framework of the cuticle; the functional integrity of the cuticular layer depends on the outer 'epicuticular' layer as well as the blend consisting of the cutin biopolymer and 'intracuticular' waxes.2 Herein, we describe a comprehensive protocol to extract waxes exhaustively from commercial tomato (Solanum lycopersicum) fruit cuticles or to remove epicuticular and intracuticular waxes …