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Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 1 Of 2, George Mcnamara
Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 1 Of 2, George Mcnamara
George McNamara
Timelapse data - GM imaging cytometer needs stitching part 1 of 2
Imaging challenge: align multiple time series over time to overcome "stage slop" in the imaging cytometer used in this experiment.
http://works.bepress.com/gmcnamara/53 (this)
and
http://works.bepress.com/gmcnamara/54 (next)
are zip files containing 8-bit stacks (MetaMorph multiplane TIFF files) acquired on an imaging cytometer.
"53" is the brightfield data, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6).
"54" is GFP fluorescence, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). The GFP fluorescence decreases over time due to photobleaching. GFP disappears from cells when they die (soluble GFP diffusing …