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Articles 1 - 15 of 15
Full-Text Articles in Life Sciences
Nitrate Removal And Patterns Of Ph-Induced Flocculation In High-Lipid Producing Algae Ochromonas Danica, Abigail E. Armstrong, Timothy Gsell Dr.
Nitrate Removal And Patterns Of Ph-Induced Flocculation In High-Lipid Producing Algae Ochromonas Danica, Abigail E. Armstrong, Timothy Gsell Dr.
Research Days
Algae have grown in popularity as a source for biofuels as some algae species, like Ochromonas danica, can produce energy-rich lipids for biofuels while simultaneously removing nutrients from wastewater. As such, use of biologic systems to mitigate pollutants are an attractive method of pollution control. Given the unique metabolic properties of O. danica, this organism is a promising candidate organism for this purpose. This study seeks to characterize the basic metabolic parameters of these algae as well as their population behaviors with attention to their growth rates and nitrate removal at varying pH over time. To do this, live cultures …
Preservation Of Ozark Glades: Soil Inocula And Plant Additions Effect On Arbuscular Mycorrhizal Fungi, Srikavi Premnath
Preservation Of Ozark Glades: Soil Inocula And Plant Additions Effect On Arbuscular Mycorrhizal Fungi, Srikavi Premnath
Show Your Stripes Research and Creative Showcase
Soil microbial communities can play a major role in the structure of natural plant communities, and restoring the soil microbial content can be important in restoring the plant community in prairies. In 2014, an experiment was set up to test whether soil inoculation along with native plant additions (Schizachyrium scoparium and Rudbeckia missouriensis) produce a similar restoration effect among glades, rocky grasslands found in south central Missouri. The following six inoculation treatments were set up in three sites: live inocula soil from well-restored glade sites, sterilized glade soil as a control, inoculated S. scoparium, uninoculated S. scoparium, inoculated R. missouriensis, …
The Relationship Between Diet And Evolution In Primates, Tori Spencer
The Relationship Between Diet And Evolution In Primates, Tori Spencer
Show Your Stripes Research and Creative Showcase
This literature review investigates the evolutionary relationship between diet and primate physiology, using both human and non-human primates as evidence of past and present examples. Special attention is devoted to physiological factors shaped by diet that have and may still cause differences between primate groups. Diet has heritable markers and can predisposition individuals to change both physically and behaviorally. Diet in primates appears to play a role in the teeth placement, brain size, shape and chemistry, taste and olfactory sense, to name a few. This information can be used to identify future human features that may continue to evolve as …
Selecting Phycoremediators For Consortia, Tori Spencer
Selecting Phycoremediators For Consortia, Tori Spencer
Show Your Stripes Research and Creative Showcase
Bioremediation is the process by which organisms remove and transform toxic compounds in a contaminated source. This is a waste management technique currently used to clean up man-made contamination. Many organisms act as natural remediators; in the case of algae, they are called phycoremediators who perform phycoremediaton. The term algae encompasses a variety of taxonomic groups and their diversity is still being realized; there are over 44,000 named species of the 72,000 species that we have identified. Some estimates calculate that there are over 200,000 diatom species alone. This diversity continues to be difficult to organize, which is problematic for …
Imaging Fluorescent Proteins In Plant Cells: A 12-Week Research-Project Oriented Cell Molecular Biology Laboratory, Michael Grubb, Bridget Mbula
Imaging Fluorescent Proteins In Plant Cells: A 12-Week Research-Project Oriented Cell Molecular Biology Laboratory, Michael Grubb, Bridget Mbula
Show Your Stripes Research and Creative Showcase
When plants are attacked by pathogenic fungi, plants defend themselves by "chewing" the fungal cell wall. Chitin, a main component of the fungal cell wall, is released and recognized by the plant cells to activate the innate immune response in the whole plant. The objective of this study is to investigate the cellular localization of the proteins encoded by two chitin-induced genes, Annexin 1 (ANN1) and SNAP33, from the genetic model Arabidopsis thaliana. The full-length cDNA (excluding stop codon) of ANN1 and SNAP33 were amplified from cDNA template and cloned into a binary vector pMDC83 to form a translational fusion …
The Relationship Between Insect Visitors And Chemical Fragrance Composition Of Cypripedium Parviflorum Var. Pubescens In Central Virginia., Ruth Elkins
Student Scholar Showcase
Due to its rarity, little is known regarding which insects pollinate Cypripedium parviflorum var. pubescens, large yellow lady slipper orchids, and why they do so when offered no reward. Insect identification and fragrance composition data have been collected from two populations of large yellow lady slippers in Central Virginia for more than a decade. This research analyzed data in order to determine potential relationships between visiting insects and fragrance compositions of the orchid. The results of this research can be used to establish and protect populations of Cypripedium parviflorum var. pubescens.
Alpha Mangostin As A Chemoprotective Agent Via Activation Of The P53 Pathway For Breast Cancer, Vanessa Van Oost
Alpha Mangostin As A Chemoprotective Agent Via Activation Of The P53 Pathway For Breast Cancer, Vanessa Van Oost
Scholar Week 2016 - present
Breast carcinoma is the most frequently diagnosed cancer among women and causes over 400,000 deaths yearly worldwide. Current treatments such as chemotherapy are not selective for cancerous tissues but are destructive to normal tissues as well. This causes a range of side effects including pain, nausea, hair loss, weakness, and more. Inactivation of p53 is an almost universal mutation within human cancer cells. The ability to activate the p53 pathway which protects cells from tumor formation is lost in 50% of cancers. Due to the prevalence of this mutation, p53 is a uniquely valuable target for applied research. Alpha mangostin …
Comparative Methodology For Analyzing Nitric Oxide Production In Raw 264.7 Murine Macrophages In Response To Lipopolysaccharide Treatment, Cassandra Robertson
Comparative Methodology For Analyzing Nitric Oxide Production In Raw 264.7 Murine Macrophages In Response To Lipopolysaccharide Treatment, Cassandra Robertson
Student Scholar Showcase
The purpose of this experiment is to develop comparative methodology for analyzing RAW 264.7 murine macrophage responses to bacterial lipopolysaccharide (LPS). These results will yield two endpoints relative to nitric oxide production: nitrites in solution from the Greiss assay, and protein expression of inducible nitric oxide synthase (iNOS). The RAW 264.7 cell line is a standard culture model of macrophage activity and stimulation with LPS induces inflammatory reactions. This process has evolved to kill ingested pathogens and activate local immune responses, including the production of nitric oxide, synthesized by iNOS. RAW 264.7 cells are stimulated with LPS doses from 1 …
A Chemical In Filter Paper Interferes With Trail Following Behavior In The Termite Reticulitermes Hesperus (Banks), Kathryn Vest
A Chemical In Filter Paper Interferes With Trail Following Behavior In The Termite Reticulitermes Hesperus (Banks), Kathryn Vest
Student Scholar Showcase
Termites follow chemical trails. In previous experiments we dissolved and diluted chemicals in acetone to test if they elicited trail following. Each trail was a straight line across the arena drawn with 10 µl using a glass capillary (Microcaps). We noticed a minor but stable response to trails laid with pure acetone. The response could be caused by contaminated acetone or by a chemical in the filter paper (Watman #5) that lined our arena (petri dish, 10cm diameter). We could confirm that the termites respond to acetone alone. Soxkhlet extraction for 8 hours removed the signal from the filter paper. …
Co-Occurrence Of Manganese Oxidase Genes Indicates Lateral Transfer Between Classes Of Proteobacteria, Jacob Olichney
Co-Occurrence Of Manganese Oxidase Genes Indicates Lateral Transfer Between Classes Of Proteobacteria, Jacob Olichney
Student Scholar Showcase
Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of 1e-50, limiting gene homologs to those with high sequence similarity. The abundance of homologous genes across classes of proteobacteria point …
Effect Of Environmental Conditions On Biomass Of Symplocarpus Foetidus, Christy Lemay
Effect Of Environmental Conditions On Biomass Of Symplocarpus Foetidus, Christy Lemay
Student Scholar Showcase
Symplocarpus foetidus (skunk cabbage) is an obligate wetland plant that occurs sporadically in wetlands throughout Virginia. Its life cycle is well studied with flowers budding in late January to early February through a process known as thermogenesis that heats the soil surrounding the plant. Pollination of fruits by flies and distribution of fruits/seeds through wildlife. Leaves appear in March with full growth above ground by early May. The leaves die back by July. Reproduction and distribution is sensitive to disturbance and environmental factors such as soil moisture, hydrology, and seed distribution. We collected environmental data in eight designated wetland plots …
Pregnancy In Zoo-Managed African Elephants (Loxodonta Africana): Investigating Possible Hormonal And Metabolic Indicators Of Successful And Unsuccessful Pregnancies., Chelsi Marolf
UNO Student Research and Creative Activity Fair
Female African elephants (Loxodanta africana) in zoos in the United States currently have high rates of ovarian acyclicity and if they do become pregnant, the calf is often lost before or during birth. This issue likely stems from various metabolic and reproductive factors, including obesity, advanced age, and stress. While this is a complicated problem, analyzing certain hormones related to reproduction and nutrition in elephants might reveal biological indicators for successful and unsuccessful pregnancies in African elephants. The goal of this study is to create a range of hormone levels throughout a typical successful pregnancy, and to compare …
Isolating And Characterizing Novel Bacteriophages From Freshwater Samples, Mackenzie Conrin, Avery Misfeldt
Isolating And Characterizing Novel Bacteriophages From Freshwater Samples, Mackenzie Conrin, Avery Misfeldt
UNO Student Research and Creative Activity Fair
Bacteria are responsible for many Healthcare-Associated infections each year and are evolving to become multidrug resistant (MDR). Bacteriophages, also called phages, are naturally occurring bacterial viruses. These phages infect and kill their bacterial hosts. It is estimated that there are 10 different phages, per bacterium. This makes phages the most abundant entity in the biosphere. Isolating and characterizing these naturally occurring bacterial killers offers the potential for strain specific treatment of MDR infections. Such treatments, coined Phage Therapy, can be used in the fight against evolving bacterial infections. Pseudomonads are a classification of bacteria that infect a variety of plants …
Effects Of Rangeland Management On Milkweed Grazing And Monarch Conservation, Brittany Poynor
Effects Of Rangeland Management On Milkweed Grazing And Monarch Conservation, Brittany Poynor
UNO Student Research and Creative Activity Fair
Cattle typically avoid consuming milkweed plants that contain high levels of toxic defense compounds, and therefore many people assume cattle avoid all milkweeds. However, common milkweed (Asclepias syriaca) contains only moderate levels of toxic compounds, and observations suggest that cattle regularly consume common milkweed and may even preferentially graze flowers and leaves of this species. These observations directly relate to efforts to add over one billion stems of milkweed to the central USA for monarch butterfly (Danaus plexippus) conservation. If cattle intentionally consume common milkweed and other milkweed species, and if certain management practices can reduce …
Exploring The Ipf Lung Through The Lens Of Single Cell Rna Sequencing, Taylor Adams, Jonas Schupp
Exploring The Ipf Lung Through The Lens Of Single Cell Rna Sequencing, Taylor Adams, Jonas Schupp
Yale Day of Data
This poster illustrates the differences between the IPF disease-specific variety of lung macrophages and the two varieties of macrophages known to reside in the normal human lung.