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Full-Text Articles in Life Sciences
Computational Analyses Of Mrna Ribosome Loading In Arabidopsis Thaliana, Joseph Benjamin Ernest
Computational Analyses Of Mrna Ribosome Loading In Arabidopsis Thaliana, Joseph Benjamin Ernest
Doctoral Dissertations
Translation of mRNA into protein is a critical step in gene expression, but the principles guiding its regulation at the genome level are not completely understood. Translation can be quantified at a genome scale by measuring the ribosome loading of mRNA—the extent to which mRNA is associated with ribosomes. In this dissertation, I present investigations into how genome-wide ribosome loading is controlled in Arabidopsis thaliana. In chapter 1, I give an overview of regulation of ribosome loading and translation. In chapter 2, I present research demonstrating for the first time that genome-wide ribosome loading in plants is partially controlled by …
Metabolomics Approaches To Decipher The Antibacterial Mechanisms Of Yerba Mate (Ilex Paraguariensis) Against Staphylococcus Aureus And Salmonella Enterica Serovar Typhimurium, Caroline Sue Rempe
Metabolomics Approaches To Decipher The Antibacterial Mechanisms Of Yerba Mate (Ilex Paraguariensis) Against Staphylococcus Aureus And Salmonella Enterica Serovar Typhimurium, Caroline Sue Rempe
Doctoral Dissertations
The increasing prevalence of drug-resistant pathogens is an urgent problem that requires novel methods of bacterial control. Plant extracts inhibit bacterial pathogens and could contain antibacterial compounds with novel mechanisms of action. Yerba mate, a common South American beverage made from Ilex paraguariensis, has antibiotic activity against a broad range of bacterial pathogens. In this work, an attempt was first made to characterize the antibacterial source of an aqueous yerba mate extract by generating a series of extract fractions, collecting GC-MS and antibacterial activity profiles, and then ranking the hundreds of compounds by their presence in fractions with high antibacterial …
Assessment Of Next Generation Sequencing Technologies For De Novo And Hybrid Assemblies Of Challenging Bacterial Genomes, Sagar Mukund Utturkar
Assessment Of Next Generation Sequencing Technologies For De Novo And Hybrid Assemblies Of Challenging Bacterial Genomes, Sagar Mukund Utturkar
Doctoral Dissertations
In past decade, tremendous progress has been made in DNA sequencing methodologies in terms of throughput, speed, read-lengths, along with a sharp decrease in per base cost. These technologies, commonly referred to as next-generation sequencing (NGS) are complimented by the development of hybrid assembly approaches which can utilize multiple NGS platforms. In the first part of my dissertation I performed systematic evaluations and optimizations of nine de novo and hybrid assembly protocols across four novel microbial genomes. While each had strengths and weaknesses, via optimization using multiple strategies I obtained dramatic improvements in overall assembly size and quality. To select …
Computational Perspective For Developing Bioluminescent Yeast Estrogen Screens For Environmental Toxicology, Jun Wang
Doctoral Dissertations
The impact of endocrine disruptive chemicals to human and wild life health has raised serious public health concerns through the past decades. To address this concern, much research was involved to develop tools for screening and assessing the hormonal potential of these compounds. Yeast bioluminescent bioreporter assay was one of the tools developed as the result of these past research endeavors. In this dissertation, a yeast bioluminescent bioreporter assay system was evaluated for the screening of endocrine disruptors from both experimental and computational perspectives. The yeast bioluminescent bioreporters were first standardized and applied in the comparative study of traditional activated …
Computational Identification Of Terpene Synthase Genes And Their Evolutionary Analysis, Qidong Jia
Computational Identification Of Terpene Synthase Genes And Their Evolutionary Analysis, Qidong Jia
Doctoral Dissertations
Terpenoids, the largest and most structurally and functionally diverse class of natural compounds on earth, are mostly synthesized by plants to be involved in various plant environment interactions. Some terpenoids are classified as primary metabolites essential for plant growth and development. Terpene synthases (TPSs), the key enzymes for terpenoid biosynthesis, are the major determinant of the tremendous diversity of terpenoid carbon skeletons. The TPS genes represent a mid-size family of about 30-100 functional genes in almost all major sequenced plant genomes. TPSs are also found in fungi and bacteria, but microbial TPS genes share low levels of sequence similarity and …