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Genomic Tools And Models For Investigating The Role Of Germline Diversity In Mouse Antibody Repertoire Development., Justin T. Kos
Genomic Tools And Models For Investigating The Role Of Germline Diversity In Mouse Antibody Repertoire Development., Justin T. Kos
Electronic Theses and Dissertations
Given the diversity and complexity within immunoglobulin (IG) loci, effective mouse models first require characterization of intra-strain differences and construction of high-quality reference assemblies for IG loci in several representative strains. To understand light chain germline diversity across biomedically significant mouse strains, we profiled the expressed IGK and IGL repertoires of 18 commonly used laboratory mouse strains using AIRR-seq. Across strains, we observed germline IGKV sequences shared by three different IGK haplotypes and a more conserved IGLV germline repertoire among common laboratory strains. Pacific Biosciences (PacBio) Single-Molecule Real-Time (SMRT) sequencing was used to sequence and assemble bacterial artificial chromosomes (BAC) …
Discovering The Pathways And Go Terms Associated With Mettl3 Modified Circular Rnas In The Embryonic Cerebral Cortex Of Mice., Dunia Zedan
Electronic Theses and Dissertations
Circular RNAs (cirRNAs) are a class of RNA molecules that result from the alternative back-splicing events that join the 3’ and 5’ ends normally present in the linear RNA molecules. It has been published that cirRNAs can function as gene regulators and as “microRNA sponges” to negatively control the functions of microRNAs. While many studies have been conducted to understand the regulatory roles of Mettl3 in linear messenger RNAs, fewer contributions were applied to understand the impact of Mettl3 modified cirRNAs on gene expression and on the regulation of different KEGG biological pathways and GO terms. This thesis was conducted …