Life Sciences Commons^™

Secretoryfactors in animals play an important role in communication between different cells, tissues and organs. Especially, the secretoryfactors with specific expression in one tissue may reflect important functions and unique status of that tissue in an organism. In this study, we identified potential tissue-specificsecretoryfactors in the fat, muscle, heart, lung, kidney and liver in the mouse by analyzing microarray data from NCBI's Gene Expression Omnibus (GEO) public repository and searching and predicting their subcellular location in GeneCards and WoLF PSORT, and then confirmed tissue-specific expression of the genes using semi-quantitative PCR reactions. With this approach, we confirmed 11 lung, 7 …

Increase of fat cells (FCs) in adipose tissue is attributed to proliferation of preadipocytes or immature adipocytes in the early stage, as well as adipogenic differentiation in the later stage of adipose development. Although both events are involved in the FC increase, they are contrary to each other, because the former requires cell cycle activity, whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis. In order to explore the important cell cycle inhibitors and study their expression in adipogenesis, we adopted a strategy combining the Gene Expression Omnibus (GEO) database available …

Background: It is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.

Results: A total of 366 unique miRNA …

Tibetan chicken is one of those chicken breeds that could adapt to high altitude and low oxygen pressure environment; it owes to an integrative genetic mechanism for hypoxia adaptability compared to lowland chicken breeds. HIF-1α and HIF-2α are the central factors playing important roles in maintaining organisms’s oxygen homeostasis. In this study, the expression of HIF-1α and HIF-2α genes were investigated by Real Time-PCR in Tibetan chicken and Dwarf chicken embryo brain tissue under normoxia (21% oxygen concentration) and hypoxia (13% oxygen concentration). The results showed that Tibetan chicken always had lower mortality than Dwarf chicken during the incubation. We …