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High-Throughput Sequencing Pipeline Tool Analysis - Staphylococcus Aureus, Justin Fay
High-Throughput Sequencing Pipeline Tool Analysis - Staphylococcus Aureus, Justin Fay
UNO Student Research and Creative Activity Fair
In this project, I compared the tools used at each step of the high-throughput sequencing pipeline used to detect single-nucleotide polymorphisms (SNP’s). There are 11 sequences I utilized from my mentor, Dr. Kate Cooper, on this project (pertaining to Staphylococcus aureus) and I used those in the sequencing pipeline. I evaluated different tools by using different ones at different steps of the pipeline. By doing this, I was able to see how close or far the outputs are by only changing one factor. It is also important to note that, with each online tool comes its own sets of parameters. …