Life Sciences Commons^™

The Genetics Of Skin Cancer: What Genes Drive The Development Of Basal Cell Carcinoma, Squamous Cell Carcinoma, And Melanoma?, Cassandra Poole, Abagail Pack, Elizabeth Whitehead, Virginia Marshall

Spring Showcase for Research and Creative Inquiry

Skin cancer is one of the most common forms of cancer worldwide. The American Academy of Dermatology estimates that 9500 people in the United States are diagnosed with skin cancer every day, and that 1 in 5 Americans will be diagnosed with skin cancer by age 70. With such a high prevalence of disease, understanding how skin cancer develops and how it can be treated is extremely important. This project aims to analyze the genes involved in the development of the three most common forms of skin cancer: basal cell carcinoma, squamous cell carcinoma, and melanoma.

A Bacterial Microbiome Analysis Of Solarized Ginseng Garden Soils, Anka Colo

Undergraduate Student Research Internships Conference

American ginseng (Panax quinquefolius) is a highly valued perennial crop grown for its roots during a four-year cultivation cycle. American ginseng is subject to ginseng replant disease (GRD) in which severe root rot develops in newly planted ginseng grown in a former ginseng garden. A common strategy to mitigating GRD is not available and techniques such as fumigation, fungicides, and biocontrol are ineffective, banned, or are slowly being phased out. Alternatively, soil solarization is a pre-plant technique used to treat soil to reduce disease inoculum and alter soil microbiomes. In summer 2019, a six-week soil solarization experiment was …

Ingredient Classification Using Food Ontology, Ricky Flores

UNO Student Research and Creative Activity Fair

A food label provides some of the most crucial information for a food product. The food label is a key resource for many health-conscious consumers for understanding ingredients. It is also vital for individuals to avoid food allergens or help patients follow dietary recommendations. While the food labels in the United States are regulated by the Food and Drug Administration (FDA) many labels contain additional information or statements that are not regulated. Moreover, the food label may be complex or contain terminology that the layperson may not understand. Evidence has indicated that consumers often find nutrition labels confusing, especially when …

Using Bioenergetics To Model The Effects Of Climate Change On Bluntnose Minnows Pimephales Notatus In A Western Kentucky Stream, Christian Slone, Christian Slone

Student Scholarship & Creative Works

Bioenergetics considers a variety of factors like consumption, excretion, and metabolism to quantify the energy use of an organism. A common use of bioenergetics modeling is to help solve fish stock problems. This project looks to develop a bioenergetics model for Bluntnose Minnows Pimephales notatus to help predict effects of climate change on the biodiversity of western Kentucky streams. We used R and NetLogo to create an agent-based bioenergetics model to simulate the size distribution of Bluntnose Minnows under various temperature regimes. Bluntnose Minnow metabolism increases, and consumption decreases with an increase in water temperature. Fish growth seems to be …

Progress Presentation On The Proposed Function Of Protein 4diu Through Bioinformatics Analyses, Melissa Hoff

Biochemistry and Molecular Biology Presentations

The BASIL project is a scientific inquiry project that aims to determine the function of a protein from its structure. Biochemistry students completed wet lab work, while structural biology students worked in silico. The purpose of this study was to determine the function of the protein with PDB ID: 4DIU. Bioinformatics analyses were completed using several databases: BLAST, Dali, Pfam, and Moltimate, and imaging of the protein was completed using PyMOL software. The BLAST database aligned the primary sequence with known protein sequences. The Dali database compared protein structure with known structures through global alignment. The Pfam database matched our …

High-Throughput Sequencing Pipeline Tool Analysis - Staphylococcus Aureus, Justin Fay

UNO Student Research and Creative Activity Fair

In this project, I compared the tools used at each step of the high-throughput sequencing pipeline used to detect single-nucleotide polymorphisms (SNP’s). There are 11 sequences I utilized from my mentor, Dr. Kate Cooper, on this project (pertaining to Staphylococcus aureus) and I used those in the sequencing pipeline. I evaluated different tools by using different ones at different steps of the pipeline. By doing this, I was able to see how close or far the outputs are by only changing one factor. It is also important to note that, with each online tool comes its own sets of parameters. …

Synthesis And Evaluation Of Novel Silica Hydride-Based Stationary Phases For Bioanalytical Application, Seiichiro Watanabe

Seiichiro Watanabe

Co-Occurrence Of Manganese Oxidase Genes Indicates Lateral Transfer Between Classes Of Proteobacteria, Jacob Olichney

Student Scholar Showcase

Pseudomonas putida GB-1 is a model organism for the study of manganese oxidation in bacteria, however, the frequency of co-localization of multiple known and suspected manganese oxidizing proteins, as well as their occurrence between species, is unknown. Eight different genes isolated from known manganese oxidizing bacteria (MnxG, MopA, McoA, PputGB1_2552, PputGB1_2553, MoxA, MofA, and Bacillus MnxG) were tested individually using BioPython and BLAST (Basic Local Alignment Search Tool) on multiple genomic databases. BLAST searches had an expect value cutoff of 1e-50, limiting gene homologs to those with high sequence similarity. The abundance of homologous genes across classes of proteobacteria point …

A Compartmental Network Model For The Spread Of Whooping Cough, Kimia Ameri

UNO Student Research and Creative Activity Fair

Outbreaks of pertussis have increased over the past few years, drawing the attention of health care providers. Understanding the transmission mechanisms of contagious disease is critically important, but depends on many intricate factors including pathogen and host environment, exposed population, and their activities. In this work, we try to improve upon the prediction model for the exposed population. The number of whooping cough reported cases in Nebraska between 2000-2017 was gathered. The standard SEIR model is used to predict the infected numbers. The results show that the Susceptible-Exposed-Infected-Recovered (SEIR) model prediction for the number of infected individuals is much higher …

Precision Medicine: Bioinformatics Assists In Finding Accurate Treatment For Her2+ Breast Cancer In Humans, Elizabeth Russman

UNO Student Research and Creative Activity Fair

Human epidermal growth factor receptor 2 (HER2) is a gene located on chromosome 17q12 (Ferrari et al., 2016). A HER2 mutation is known to cause breast cancer, and is responsible for approximately 20% of all breast cancers.

In this project, HER2 positive breast cancer will be examined. The goal of this project is to understand the complicated gene, HER2, and how treatment needs to be more individualized and precise using bioinformatics.

Analysis Of Clustering Algorithms, Ethan Summers

UNO Student Research and Creative Activity Fair

In Bioinformatics, choosing the right algorithm for a problem is very important. Choosing the wrong algorithm or one that is less efficient can make or break a project. Analyzing algorithms beforehand is key. The goal of this project is to analyze three clustering algorithms for protein protein interaction networks and compare their function and results. A clustering algorithm takes a dataset, in this case a simulated PPI (protein-protein interaction) network and groups together similar data points based on some similarity criteria. It is important to know the difference between these algorithms to get the desired results.

Data Analytics Pipeline For Rna Structure Analysis Via Shape, Quinn Nelson

UNO Student Research and Creative Activity Fair

Coxsackievirus B3 (CVB3) is a cardiovirulent enterovirus from the family Picornaviridae. The RNA genome houses an internal ribosome entry site (IRES) in the 5’ untranslated region (5’UTR) that enables cap-independent translation. Ample evidence suggests that the structure of the 5’UTR is a critical element for virulence. We probe RNA structure in solution using base-specific modifying agents such as dimethyl sulfate as well as backbone targeting agents such as N-methylisatoic anhydride used in Selective 2’-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE). We have developed a pipeline that merges and evaluates base-specific and SHAPE data together with statistical analyses that provides confidence …

Gene Co-Expression Networks Analysis Reveal Novel Molecular Endotypes In Alpha-1 Antitrypsin Deficiency, Jen-Hwa Chu, Wenlan Zang

Yale Day of Data

Rationale:Alpha-1 antitrypsin deficiency (AATD) is a genetic condition that predisposes to early onset pulmonary emphysema and airways obstruction. The exact mechanism through which AATD leads to lung disease is incompletely understood.

Objectives: To investigate the effect of AAT genotype and augmentation therapy on bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) transcriptome, while examining the link between gene expression profiles, and clinical features of AATD.

Methods: We performed RNA-Seq on RNA extracted from BAL and PBMC on samples obtained from 89 AATD patients enrolled in the Genomic Research in Alpha-1 Antitrypsin Deficiency and Sarcoidosis (GRADS) study. Differential …

Intro To Rna-Sequencing Lecture Slides, Ray A. Enke

Ray Enke Ph.D.

Rna-Seq Bioinformatics Analysis Using Cyverse Discovery Environment, Ray A. Enke

Ray Enke Ph.D.

Transcripity Split: Course-Based Rna-Seq Analysis Using The Ultrafast Kallisto-Sleuth Pipeline, Ray A. Enke

Ray Enke Ph.D.

No abstract provided.

Identification Of Genes Involved In Diauxic Shift Of Saccharomyces Cerevisiae Through Gateway Node Analysis., Emily Pachunka

UNO Student Research and Creative Activity Fair

The use of high-throughput assays, or experiments yielding large data sets, in biological research has become a standard practice in laboratories throughout the world. Because such investigations have the ability to produce high volume and comprehensive data sets, it is then important to develop methods that allow researchers to quickly pull meaningful information from an overwhelming amount of data. Network modeling has become a popular technique for visualizing and analyzing large biological data sets. A network is a basic graph with nodes and edges (i.e. social networks) that also integrates complex principles of graph theory for deeper analysis and pattern …

Correlation Networks: Causative Relationships From Gene Expression Data, Grogan W. Huff

UNO Student Research and Creative Activity Fair

Genes that share expression conditions show a biological correlation, and no modern method of visualization displays these intricate co-expression patterns better than a graph. Structural observations about a co-expression graph can reveal the secrets of the biological system that it models, but experimentally validated co-expression graphs are pain-staking work to produce. Present day correlation network analysis shows potential for drawing conclusions from large volumes of biological systems data in an inexpensive and easy-to-produce way, however, work remains to confirm the appropriateness and scope of such methods for specific, scientific application. Toward this effort, we generated a Pearson correlation network from …

A Machine Learning Approach To Post-Market Surveillance Of Medical Devices, Jonathan Bates, Shu-Xia Li, Craig Parzynski, Ronald Coifman, Harlan Krumholz, Joseph Ross

Yale Day of Data

Post-market surveillance is a collection of processes and activities used by product manufacturers and regulators, such as the U.S. Food and Drug Administration (FDA) to monitor the safety and effectiveness of medical devices once they are available for use “on the market”. These activities are designed to generate information to identify poorly performing devices and other safety problems, accurately characterize real-world device performance and clinical outcomes, and facilitate the development of new devices, or new uses for existing devices. Typically, a device is monitored by comparing adverse events in the exposed population to a matched unexposed population. This research considers …

Social Networks And Web2.0 Among Youth: Lessons For Pacific Island Nations, Deogratias Harorimana Sr

Dr Deogratias Harorimana

This study is on social networks and web2 among youths and the lessons for Pacific Island nation. This study defines commonly used social networking sites used by the Pacific youths, average time spent, reasons behind the use of social networking sites and how social networking sites can be used as a development tool for Pacific Island nation. It was found that the popularity of social networking amongst youths in Pacific Island Countries is fast growing, increasing more than three folds year on year in the last 3years. Social Networks are a vital part of life for PIC youths, where, now …

A Review Of International Best Practice In E-Governmentsome Lessons For New Adopters, Deogratias Harorimana Sr

Dr Deogratias Harorimana

Efficient bureaucratic processes as essential to attract and retain investment, as well as promote SME growth. The e_Gov is one of many ways emerging economies have used to streamline public service delivery and create a freindly and conducive atmosphere for business -both MNC and SMEs. This presentation provide an overview of some of the World's most recent case examples on the successful design-plan-implementation of eGov to build a strong basis to attract investment and deliver seamless essential services to Citizens.

An Entrepreneurial Approach To Librarianship, Flora G. Shrode, Jennifer R. Duncan, Wendy Holliday

Flora Shrode

Librarians from Utah State University explain recent efforts to encourage subject librarians to take a more holistic view of their roles. We are shifting from a traditional emphasis primarily on collection development and refocusing on natural connections between collections, instruction, liaison, and reference service. The poster provides background about Utah State University’s situation and explains our approach to analyzing local needs and culture to inform development of a new organizational structure. We describe our vision of subject librarianship, the process by which we assessed librarians’ ideas and goals for performing as subject librarians, and the actions we are taking to …

An Entrepreneurial Approach To Librarianship, Flora Shrode, Jennifer Duncan, Wendy Holliday

Library Faculty & Staff Publications

Librarians from Utah State University explain recent efforts to encourage subject librarians to take a more holistic view of their roles. We are shifting from a traditional emphasis primarily on collection development and refocusing on natural connections between collections, instruction, liaison, and reference service. The poster provides background about Utah State University’s situation and explains our approach to analyzing local needs and culture to inform development of a new organizational structure. We describe our vision of subject librarianship, the process by which we assessed librarians’ ideas and goals for performing as subject librarians, and the actions we are taking to …