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Full-Text Articles in Life Sciences

Detecting Native Freshwater Mussels In Pennsylvania Waterways: Comparison & Validation Of Environmental Dna Methods, Meredith Bennett May 2023

Detecting Native Freshwater Mussels In Pennsylvania Waterways: Comparison & Validation Of Environmental Dna Methods, Meredith Bennett

Electronic Theses and Dissertations

North America is home to approximately one third of the world's freshwater mussel species. They are highly imperiled organisms due to habitat destruction and invasive species. Traditional surveys rely on visual identification of mussels, but individuals tend to be rare and difficult to identify. An alternative method is to extract environmental DNA (eDNA) from water samples, which has advantages over traditional sampling, including less sampling effort and fewer hazards to researchers and organisms. We conducted a review of the two main eDNA approaches: single-species detection and metabarcoding. We also developed and validated metabarcoding primers for the detection of native mussels. …


Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough Aug 2022

Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough

Electronic Theses and Dissertations

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative …


Detection, Occupancy, Abundance, And Mercury Accumulation Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Texas, David Rosenbaum May 2022

Detection, Occupancy, Abundance, And Mercury Accumulation Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Texas, David Rosenbaum

Electronic Theses and Dissertations

Land use practices and physical alterations of ecosystems result in habitat loss and fragmentation, while chemical alterations, such as pollutant input, reduce habitat quality and health of exposed organisms. Here, I investigated the effects of watershed- and local-scale environmental variables on the occupancy, abundance, and mercury accumulation of a threatened aquatic species (Macrochelys temminckii, i.e., alligator snapping turtle) within the southwestern periphery of its distribution. Hierarchical modeling suggested the distribution of the species is more affected by watershed-scale land-cover than local habitat, and provided a baseline estimate of average species abundance across its range in eastern Texas. Abundance …


Approaches To Arthropod Conservation : Landscape Genetics, Community Assessment, And Prediction Of Extinction Risk., Victoria Annette Prescott Dec 2016

Approaches To Arthropod Conservation : Landscape Genetics, Community Assessment, And Prediction Of Extinction Risk., Victoria Annette Prescott

Electronic Theses and Dissertations

Although urbanization is a leading cause of species extinction throughout the world, the impact of urban development on arthropods is little studied and, as a result, poorly understood. I used three distinct approaches to studying arthropod conservation in North America. First, I used landscape genetics techniques to study the impact of urbanization on gene flow among populations of Rabidosa rabida, the rabid wolf spider. While gene flow was not detrimentally reduced, urban development correlated with a reduction in migration rates among populations, and to my knowledge, this is the first study to document isolation by resistance in spiders. Next, …