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Full-Text Articles in Life Sciences
Cell Division Regulation In Staphylococcus Aureus, Catherine M. Spanoudis
Cell Division Regulation In Staphylococcus Aureus, Catherine M. Spanoudis
USF Tampa Graduate Theses and Dissertations
Cell division is a fundamental biological process that occurs in all kingdoms of life. Our understanding of cell division in bacteria stems from studies in the rod-shaped model organisms: Gram-negative Escherichia coli and Gram-positive Bacillus subtilis. The molecular underpinnings of cell division regulation in non-rod-shaped bacteria remain to be studied in detail. Rod-shaped bacteria possess many positive and negative regulatory proteins that are essential to the proper placement of the division septa and ultimately the production of two identical daughter cells, many of which are absent in cocci. Given that essential cell division proteins are attractive antibacterial drug targets, it …
Mass Spectrometry-Based Methods For The Detection And Characterization Of Protein-Tyrosine Nitration, Kent W. Seeley
Mass Spectrometry-Based Methods For The Detection And Characterization Of Protein-Tyrosine Nitration, Kent W. Seeley
USF Tampa Graduate Theses and Dissertations
Protein tyrosine nitration (PTN) is a posttranslational modification resulting from oxidative/nitrosative stress that has been implicated in a wide variety of disease states. Characterization of PTN is challenging due to several factors including its low abundance in a given proteome, preferential site modification, multiple target site proximity within unique peptide sequences, and analytical method and instrument limitations. Current analytical techniques are insufficiently sensitive to identify endogenous nitration sites without incorporation of either nitrotyrosine or target protein enrichment. However, enrichment proficiency can also be inadequate. Chemical derivatization of the nitro- moiety can be incomplete or result in undesirable byproduct formation, while …