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Molecular Validation Of Lpxc As An Antibacterial Drug Target In Pseudomonas Aeruginosa, Adam Barb, Khisimuzi E. Mdluli, Pamela R. Witte, Toni Kline, Alice L. Erwin, Bryce E. Mansfield, Amanda L. Mcclerren, Michael C. Pirrung, L. Nathan Tumey, Paul Warrener, Christian R. H. Raetz, C. Kenall Stover
Molecular Validation Of Lpxc As An Antibacterial Drug Target In Pseudomonas Aeruginosa, Adam Barb, Khisimuzi E. Mdluli, Pamela R. Witte, Toni Kline, Alice L. Erwin, Bryce E. Mansfield, Amanda L. Mcclerren, Michael C. Pirrung, L. Nathan Tumey, Paul Warrener, Christian R. H. Raetz, C. Kenall Stover
Adam Barb
LpxC [UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc deacetylase] is a metalloamidase that catalyzes the first committed step in the biosynthesis of the lipid A component of lipopolysaccharide. A previous study (H. R. Onishi, B. A. Pelak, L. S. Gerckens, L. L. Silver, F. M. Kahan, M. H. Chen, A. A. Patchett, S. M. Galloway, S. A. Hyland, M. S. Anderson, and C. R. H. Raetz, Science 274:980-982, 1996) identified a series of synthetic LpxC-inhibitory molecules that were bactericidal for Escherichia coli. These molecules did not inhibit the growth of Pseudomonas aeruginosa and were therefore not developed further as antibacterial drugs. The inactivity of the LpxC …
Mid-Ir Biosensor: Detection And Fingerprinting Of Pathogens On Gold Island Functionalized Chalcogenide Films, Chenxu Yu, Ashtosh Ganjoo, H. Jain, C. G. Pantano, Joseph Irudayaraj
Mid-Ir Biosensor: Detection And Fingerprinting Of Pathogens On Gold Island Functionalized Chalcogenide Films, Chenxu Yu, Ashtosh Ganjoo, H. Jain, C. G. Pantano, Joseph Irudayaraj
Chenxu Yu
Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the surface of IR-transparent Ge-containing chalcogenide glass films were formed via thiol chemistry on 20-nm-thick gold islands. As a first step, the protocol was validated by monitoring fluorescently tagged targets to validate binding. FT-IR spectroscopy confirmed that the coating of the films with 20-nm gold did not have a significant effect on the propagation and penetration of IR evanescent waves through the film. The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR fingerprinting. Highly selective detection …