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Biochemistry, Biophysics, and Structural Biology
Chromatin domains/CTCF/globin genes/insulators/odorant receptors
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Full-Text Articles in Life Sciences
Structural And Functional Conservation At The Boundaries Of The Chicken Β-Globin Domain, Noriko Saitoh, Adam C. Bell, Felix Recillas-Targa, Adam G. West, Melanie A. Simpson, Michael Pikaart, Gary Felsenfeld
Structural And Functional Conservation At The Boundaries Of The Chicken Β-Globin Domain, Noriko Saitoh, Adam C. Bell, Felix Recillas-Targa, Adam G. West, Melanie A. Simpson, Michael Pikaart, Gary Felsenfeld
Department of Biochemistry: Faculty Publications
We show that the 3' boundary of the chicken β -globin locus bears striking structural similarities to the 5' boundary. In erythroid cells a clear transition in DNase I sensitivity of chromatin at the 3' end of the locus is observed, the location of this transition is marked by a constitutive DNase I hypersensitive site (HS), and DNA spanning this site has the enhancer-blocking capacity of an insulator. This HS contains a binding site for the transcription factor CTCF. As in the case of the 5' insulator, the CTCF site is both necessary and sufficient for the enhancer-blocking activity of …