Life Sciences Commons^™

Expectation Values Of Simple Correlated Helium-Atom Wavefunctions, Thomas P. Tsien, Russell T. Pack

Faculty Publications

In this Note we present the results of a calculation of the expectation values of a number of one- and two electron properties of the ground-state helium atom using two very simple correlated wavefunctions.

Membrane Bound Dehydrogenase Enzymes And Related Electron Transport Systems In Acetobacter Aceti Var. Liquefaciens: Their Induction And Pleiotropic Nature, John C. Rakoczy

Masters Theses

No abstract provided.

The Transfer Of Hydrogen In The Reduction Of Progesterone, William H. Kersey

Loma Linda University Electronic Theses, Dissertations & Projects

Experimental female rats were given daily injections of synthetic estrogens for six months. The effect on rat ovarian 20α-OH-SDH was not definitive. Diethylstilbestrol and 17α-ethynylestradiol caused a significant drop in activity as compared with controls while mestranol caused a slight increase in activity. A synthetic progestin, medroxyprogesterone, had no apparent effect on the activity of the enzyme.

Rat ovarian 20α-OH-SDH was purified fivefold by ammonium sulfate fractionation and chromatography on DEAE-cellulose.

The homogeneity of the enzyme was checked by means of disc gel electrophoresis. At pH 8.2 no significant contamination appeared in the electrophoretic pattern, but at pH 9.0 five …

Actomyosin-Like Protein Isolated From Mammalian Brain / S. Puszkin, S. Berl, Columbia University, College Of Physicians And Surgeons, New York 10032, Elena Puszkin, Mount Sinai School Of Medicine, New York, D. D. Clarke, Fordham University, Bronx, New York, S. Puszkin, Soll Berl, Elena Puszkin, Donald Dudley Clarke Phd

Chemistry Faculty Publications

A protein with characteristics similar to actomyosin has been isolated from whole brain of rat and cat. It is soluble in 0.6 molar potassium chloride and insoluble in 0.1 1nolar potassium chloride. It superprecipitates with magnesium ions and adenosine triphosphate. It has adenosine triphosphatase activity stimulated by either 1nagnesium or calcium ions. Both superprecipitation and adenosine triphosphatase activity are inhibited by p-chloromercuribenzoate and Mersalyl but not by ouabain

The Effect Of Puromycin On Protein Synthesis Of Cell Free Systems From Mesophilic And Thermophilic Bacteria, Philip Y. Shen

Masters Theses

No abstract provided.

Concentration And Fatty Acid Distribution Of Aortic Cerebrosides In Cholesterol-Fed And Normal Rabbits, Robert V. P. Tao

Masters Theses

No abstract provided.

Isolation And Partial Characterization Of The Toxic Substance From Eremocarpus Setigerus Benth, Shuliang Lee

Loma Linda University Electronic Theses, Dissertations & Projects

The work on the active substance of Eremocarpus setigerus Benth has been previously performed by Naito and Noller. However, the investigation was not complete. Therefore we isolated and tested this substance by steam distillation and thin layer chromatography technique. A yellow sticky toxic substance was isolated with Rf values of 0.15 in benzene system and 0.57 in benzene-absolute ethanol (95:5 v/v) system.

The toxic substance which is free of halogen, nitrogen, and sulfur, is soluble in ethyl ether, ethanol, and concentrated sulfuric acid. It has a maximum absorption in the ultraviolet region that indicates the presence of unsaturation. Its infrared …

Effect Of Fluoroacetate On Amino Acid Metabolism / W. J. Nicklas, D. D. Clarke, And S. Berl. Chem. Dept., Fordham Univ., And Col. Of Physicians And Surgeons, Columbia Univ., New York, N. Y., William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

Guinea pig brain cortex slices incubated in media containing U-14-aspartate or glutamate (GA) (J. Neurochem. in press) form glutamine ( GM) of relative specific activity (RSA)> 1 (GA=l). In the presence of 10-3 fluoroacetate (FA) the RSA of GM decreased to values considerably < 1 without any changes in levels of amjno acids; the % of label in GA doubled whilA that in GM decreased correspondingly. Similar results were obtained with 1-14 C-acetate as tracer in vivo (mice) and in vitro. RSA' s of Grvl > 1 have been explained by the compartmentation of GA in cerebral cortex. Consequently the effect of GA may be explained by an inhibition of the flow of metabolites through that pool of GA used preferentially for GM synthesis. This is consistent with the concept of the existence, in brain, of more than one citric acid cycle, one …

A Micro Modification Of The Automatic Determination Of Serum Iron / Donald D. Clarke Nd Ruth Nicklas, Donald Dudley Clarke Phd, Ruth Nicklas

Chemistry Faculty Publications

No abstract provided.

Fluorofumarate - A Substrate For Fumarate Hydrotase / Donald D. Clarke William J. Nicklas John Palumbo Department Of Chemistry Fordham University Bronx, New York 10458, Donald Dudley Clarke Phd, William J. Nicklas, John Palumbo

Chemistry Faculty Publications

No abstract provided.

Contractile Protein In Brain / S. Puszkin, D. D. Clarke And S. Berl. Col. Of Physicians And Surgeons, Columbia University, N.Y.C., S. Puszkin, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

Evidence suggested there might exist in brain a contractile protein: 1) actomyosin contains Nacetyl aspartic acid; brain contains a high concentration of the free compound, 2) actomyosin functions as a Mg dependent ATPase; brain contains a Mg dependent ATPase, 3) actomyosin-like contractile proteins have been described in cells, membranes, and tissues other than muscle. Therefore whole rat brains were extracted with 0.6M KCl and the extract diluted to O.lM KCl. The precipitate exhibited superprecipitation (SP) properties which required both Mg and ATP. SP was inhibited by p-chloro-mercuribenzene sulfonic acid (10- M) but not by ouabain (10 M). The protein purified …

Gas Chromatographic Determination Of Dopamine And Related Compounds / D. D. Clarke, S. Wilk, And S. E. Gitlow Chemistry Department Fordham University Bronx, New York Department Of Medicine Mount Sinai Hospital New York, New York, Donald Dudley Clarke Phd, Sherwin Wilk, Stanley E. Gitlow

Chemistry Faculty Publications

No abstract provided.

Compartmentation Of Glutamic Acid Metabolism In Brain Slices / S. Berl, W.J. Nicklas, And D.D. Clarke Department Of Neurology, College Of Physicians And Surgeons, Columbia University, Department Of Chemistry, Fordham University, New York, Soll Berl, William J. Nicklas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

(1) Compartmentation of glutamate metabolism in brain cortex previously observed only in vivo, has now been demonstrated in vitro. This was shown by using [U-14C]aspartate and [U-14C]glutamate as tracer substrates. (2) Preparation and maintenance of the slices at 0° resulted in reversible inhibition of glutamine synthesis. Preincubation at 37° for 10 min or preparation of the slices at room temperature partially overcame this inhibition. (3) Transfer to fresh medium after preincubation had an added stimulatory effect on glutamine synthesis. (4) Incubation in high K+ medium (27 mM) altered the relative specific activity of glutamine. (5) The data are in keeping …

The Function And Use Of Sulphur Dioxide In West Australian Winemaking, D Mann

Journal of the Department of Agriculture, Western Australia, Series 4

THE judicious use of sulphur dioxide is perhaps the most important of the fundamentals of winemaking in a hot climate.

Western Australia's vintage, centred in the Swan Valley just a few miles north-east of Perth, is subjected to some of the highest summer temperatures experienced by a quality wine producing district.

In this environment, it is important to make good use of sulphur dioxide to avoid wine spoilage.