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Mechanism Of Decarboxylation Of Glycine And Glycolate By Isolated Soybean Cells, David J. Oliver

David J. Oliver

Isolated soybean leaf mesophyll cells decarboxylated exogenously added [1-14C]glycolate and [1-14C]glycine in the dark. The rate of CO2 release from glycine was inhibited over 90% by isonicotinic acid hydrazide and about 80% by KCN, two inhibitors of the glycine to serine plus CO2 reaction. The release of CO2 from glycolate was inhibited by less than 50% under the same conditions. This indicates that about 50% of the CO2 released from glycolate occurred at a site other than the glycine to serine reaction. The sensitivity of this alternative site of CO2 release to an inhibitor of glycolate oxidase (methyl-2-hydroxy-3-butynoate) but not …