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Biochemistry, Biophysics, and Structural Biology
Loma Linda University Electronic Theses, Dissertations & Projects
- Keyword
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- Anogenital Cancer (1)
- Antiviral Agents -- therapeutic use (1)
- Asymmetric reactions; Assymetric bonds; Organocatalysts (1)
- Cervical Cancer (1)
- Chemoresistance (1)
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- DNA Damage (1)
- Development Gene Transcription; Noncoding DNA; Morphogenesis; CNR-Green flourescent protein; Fluorescent Microscopy Localization; (1)
- Fetal Intrauterine Growth Restriction; Uterine Vascular Adaptation; Basal Uterine Vascular Tone; (1)
- Gemcitabine Therapy; Pancreatic Cancer; Chemotherapy; Radiotherapy; Survivin Transcription (1)
- Gene Expression (1)
- HIV-1 -- drug effects (1)
- Human Papillomavirus 16 (1)
- Human Papillomavirus; Cervical cancer; Carcinogenesis; Viral integration; Virus-mediated factors; Cancer susceptibility (1)
- Maternal hypoxia; Perinatal hypoxic-ischemic encephalopathy; Systematic asphyxia; Organogenesis (1)
- Oral and Pharyngeal Cancers (1)
- Oxidative Stress (1)
- Pancreatic Cancer; Cancer Therapy Resistance; Tumor Microenvironment; Intracellular Adaptations; Resistance-linked Genes (1)
- Preconditioning; Endogenous Protective Mechanisms; Surgical Brain Injury Rodent Model; Endogenous Fibrinogen; Perioperative Hemorrhage; Postoperative Brain Edema (1)
- RNA Sequencing (1)
- RNA, Catalytic -- therapeutic use (1)
- Serum-derived Exosomes; Prostate cancer; Stress survival proteins (1)
- Stem cell-based regenerative therapy; Myocardial regeneration; Endogenous Cardiovascular Progenitor Cells; (1)
- Survivin-T34A; Pancreatic Cancer; Cancer Fatality; Anti-metabolity Agent; Gemcitabine; Genotoxic Stress (1)
- Survivin; Tumor Microenvironment; Extracellular Milieu; Phenotypic Changes (1)
- T Cell Suppression; Myeloid-derived Suppressor cells; Natural Killer (NK) Cells; Survivin; B Cell Lymphoma Exosomes; (1)
- Traumatic Brain Injury; Concussions (1)
- Virus-Induced Mutagenesis (1)
Articles 1 - 30 of 97
Full-Text Articles in Life Sciences
Investigating The Role Of The Three N-Terminal Hamp Domains Of The Aer2 Chemoreceptor From Pseudomonas Aeruginosa, Selina Anaya
Investigating The Role Of The Three N-Terminal Hamp Domains Of The Aer2 Chemoreceptor From Pseudomonas Aeruginosa, Selina Anaya
Loma Linda University Electronic Theses, Dissertations & Projects
Pseudomonas aeruginosa is a ubiquitous environmental bacterium and opportunistic human pathogen. It readily adapts to changing environmental conditions with the aid of 26 chemoreceptors that signal to four distinct chemosensory systems. The Aer2 chemoreceptor from P. aeruginosa is an O2 sensor implicated in stress responses, virulence and fine-tuning the chemotaxis (Che) system. Aer2 is the sole chemoreceptor of the Che2 chemosensory system, which forms complexes at the cell pole during stationary phase. Aer2 is soluble, yet membrane-associated and displays an unusual arrangement with its PAS sensing domain sandwiched between three N-terminal and two C-terminal HAMP domains. HAMP domains are widespread …
Signaling Pathways In Skeletal Development And Metabolism, Richard Charles Lindsey
Signaling Pathways In Skeletal Development And Metabolism, Richard Charles Lindsey
Loma Linda University Electronic Theses, Dissertations & Projects
Osteoporosis, a disease characterized by low bone mass and microarchitectural deterioration, causes nine million debilitating fractures annually. An increase in bone resorption not adequately compensated by a corresponding increase in bone formation is the major cause of osteoporosis. Since current therapies have failed to effectively eliminate osteoporosis, there is an urgent need to discover novel mechanisms regulating bone resorption and formation and develop new therapeutic approaches to treat this disease. To that end, we have explored novel, non-canonical signaling mechanisms of two well-established regulators of bone: thyroid hormone (TH) and vitamin C (ascorbic acid, AA). Since previous studies have shown …
A Novel Exo-Proteomic Approach To The Study Of Traumatic Brain Injury, Ron B. Moyron
A Novel Exo-Proteomic Approach To The Study Of Traumatic Brain Injury, Ron B. Moyron
Loma Linda University Electronic Theses, Dissertations & Projects
Concussions and Traumatic Brain Injuries (TBI) are significant health concerns and affect a wide cross section of society. Current diagnostic criteria and modalities, such as brain imaging and subjective measures of consciousness such as the Glasgow Coma Scale (GCS) score, are insufficient to properly diagnose the full spectrum of head injuries. Assessment of injury severity and outcome are further complicated by the vast array of symptoms, many of which mimic those displayed by other disorders. It is important to possess a better diagnostic tool for head injury triage and outcome prediction. One current line of inquiry seeks to discover a …
Rna-Seq Reveals Transcriptomic Program Associated With Stemness In Taxane Resistant Prostate Cancer, Christina K. Cajigas-Du Ross
Rna-Seq Reveals Transcriptomic Program Associated With Stemness In Taxane Resistant Prostate Cancer, Christina K. Cajigas-Du Ross
Loma Linda University Electronic Theses, Dissertations & Projects
There is no cure for advanced prostate cancer (PCa), and taxane chemotherapy is the only treatment option once other therapies have failed. However, this is problematic since all patients eventually develop chemoresistance. Emerging treatments for advanced PCa have shown promise at the benchside, but clinical trials have not resulted in newly approved drugs due in part to redundant survival pathways utilized by prostate tumor cells to maintain therapy-resistance. Using RNAsequencing—an innovative approach for quantifying gene expression changes—this dissertation sought to elucidate chemoresistance-associated molecular pathways as a catalyst to develop new therapeutic targets. Results revealed a differential upregulation of stemness-associated genes …
Aryl Hydrocarbon Receptor Mediated Restoration Of Cytoglobin As A Treatment For Breast Cancer, Leah Kathleen Rowland
Aryl Hydrocarbon Receptor Mediated Restoration Of Cytoglobin As A Treatment For Breast Cancer, Leah Kathleen Rowland
Loma Linda University Electronic Theses, Dissertations & Projects
Nearly 40,000 women die annually from breast cancer in the US. Clinically available targeted breast cancer therapy is ineffective in triple negative breast cancer (TNBC) subtypes, which lack expression of the estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2. TNBC carries a poor prognosis. Previous reports show that aryl hydrocarbon receptor (AhR) agonist 2-(4-amino-3-methylphenyl)- 5-fluorobenzothiazole (5F 203) selectively inhibits the growth of breast cancer cells including those of the TNBC subtype. In the current study, we determined that 5F 203 induces the expression of epigenetically silenced putative tumor suppressor gene cytoglobin (CYGB) and suppresses TNBC cell growth …
Pain And Stress In The Premature Infant, John Bryle Chong Tan
Pain And Stress In The Premature Infant, John Bryle Chong Tan
Loma Linda University Electronic Theses, Dissertations & Projects
Procedural pain and stress in premature neonates is currently assessed utilizing tools that lack objectivity and accuracy. This results in untreated or mismanaged pain. My dissertation utilized physiological methods and biochemical markers that identified pain in premature neonates and its effect on energy metabolism. The painful procedure that I examined is the retinopathy of premature (ROP) examination, a necessary and routine eye examination in the neonatal intensive care unit (NICU) that detects and identifies infants at risk for blindness due to retinal detachment. The effects of the ROP exam on peripheral and deep tissue oxyhemoglobin saturation and urinary purine degradation …
Effect Of Extracellular Survivin And Lymphoma Exosomes On Natural Killer Cells, Heather R. Ferguson Bennit
Effect Of Extracellular Survivin And Lymphoma Exosomes On Natural Killer Cells, Heather R. Ferguson Bennit
Loma Linda University Electronic Theses, Dissertations & Projects
Tumors alter their microenvironment to promote survival using methods such as angiogenesis promotion, growth signals, and immune suppression. The immune system becomes unresponsive to transformed neoplastic cells through a variety of methods including T cell suppression, increased myeloid-derived suppressor cells (MDSCs), and reduced natural killer (NK) cell activity. NK cells have inherent killing capabilities and thus are among the first responders in recognizing and destroying abnormal cells. However, many types of cancers inhibit the surveillance and cytotoxic abilities of NK cells by releasing exosomes, vesicles that can modulate the tumor microenvironment (TME) and intercellular communication for the purpose of enhancing …
A Novel Population Of Cardiovascular Progenitors Persist In Neonates As Mesendodermal Cells, Julia Kim
A Novel Population Of Cardiovascular Progenitors Persist In Neonates As Mesendodermal Cells, Julia Kim
Loma Linda University Electronic Theses, Dissertations & Projects
The rise in mortality due to cardiovascular disease has increased the need to develop an efficient regenerative therapeutic for heart failure. Numerous cell-based therapies have been investigated for myocardial regeneration; however, an optimal progenitor has yet to be discovered. Identifying a resident cell population with enhanced ability to differentiate into multiple lineages would greatly contribute to the field of stem cell-based regenerative therapy. Evidence suggests that endogenous cardiovascular progenitor cells (CPCs) that have been isolated from the heart itself express ISL1, KDR, and MESP1, and are capable of differentiating into all major cardiac lineages. The earlier developmental stage at which …
Synthesis Of Huacat® Analogues As Novel Organocatalysts For The Formation Of Asymmetric C-C Bonds, Kenneth Laboy
Synthesis Of Huacat® Analogues As Novel Organocatalysts For The Formation Of Asymmetric C-C Bonds, Kenneth Laboy
Loma Linda University Electronic Theses, Dissertations & Projects
The synthesis of organic compounds with preferential stereochemistry is ubiquitous in the scientific community. A chemical reaction that asymmetrically induces one stereoisomer over another is achieved through the use of catalysts and various coupling reagents.1 Inorganic catalysts, such as TiCl4 and ZnCl2, have been documented for many decades as effective agents in the synthesis of asymmetric bonds, however, there are environmental limitations to their use. First, the asymmetric reactions involving these metal catalysts require solvents that are not environmentally-friendly, especially when used on an industrial scale and second, the metal catalysts are recycled when possible, however, there is always metal …
Proteomic Profiling Of Serum Derived Exosomes From Prostate Cancer Patients, David Turay
Proteomic Profiling Of Serum Derived Exosomes From Prostate Cancer Patients, David Turay
Loma Linda University Electronic Theses, Dissertations & Projects
Touted among the major achievements in the diagnosis and management of Prostate cancer (PCa) in the past few decades has been, the dramatic decline of men with advanced/metastatic PCa at diagnosis coupled with a significant improvement ( >90%) in the five and ten year survival rates of the disease. Non-palpable PCa (potentially clinically treatable disease) now accounts for 70-80% of all newly diagnosed cases of PCa. Preceding these changes by about a decade was the introduction of Prostatic Specific Antigen (PSA) into clinical practice; first as biomarker for monitoring response to therapy and subsequently as a complementary screening tool. It …
Factors Promoting Human Papillomavirus Mediated Cervical Carcinogenesis, Yan Chen Wongworawat
Factors Promoting Human Papillomavirus Mediated Cervical Carcinogenesis, Yan Chen Wongworawat
Loma Linda University Electronic Theses, Dissertations & Projects
High-risk human papillomavirus (HPV) is the causative agent of cervical cancer. Integration of the HPV genome into the host genome is a key event in cervical carcinogenesis, with oxidative stress (OS) likely playing a major role in promoting DNA damage, and subsequently, integration. In our current study, we demonstrated a chain of events leading from the induction of OS, to DNA damage, and then to viral integration. Induction of OS by either virus-mediated factors, such as expression of E6*, a splice variant of the E6 oncogene, or by exogenous factors led to DNA damage in normal oral keratinocytes and in …
Crotalus Snake Venom Preconditioning To Prevent Surgical Brain Injury, Cherine Hee-Sun Kim
Crotalus Snake Venom Preconditioning To Prevent Surgical Brain Injury, Cherine Hee-Sun Kim
Loma Linda University Electronic Theses, Dissertations & Projects
Preventive measures are increasingly relevant to medical practice. Preconditioning, a preemptive therapy that administers mildly harmful stimuli to induce endogenous protective mechanisms before major injury, has been shown to minimize injury in many animal models. Given the elective nature of most neurosurgical procedures, the surgical brain injury (SBI) rodent model provides an ideal platform for preconditioning. Our work shows that preconditioning with Crotalus rattlesnake venom, known for its hemorrhagic and inflammatory effects, mitigates some harmful effects of SBI. We have identified two proteins of interest in Crotalus venom: snake venom metalloproteinase (SVMP), an enzyme with hemorrhagic effects, and phospholipase A2 …
Curcumin: A Multi-Dimensional Approach To Pancreatic Cancer Targeting Cell Death And Exosomes, Carlos J. Diaz Osterman
Curcumin: A Multi-Dimensional Approach To Pancreatic Cancer Targeting Cell Death And Exosomes, Carlos J. Diaz Osterman
Loma Linda University Electronic Theses, Dissertations & Projects
Pancreatic cancer is currently one of the most difficult diseases to treat due to difficulty of detection and the aggressive nature of the disease. In addition, pancreatic cancer has the highest mortality rates compared to other cancer types. These mortality rates are attributable in part to increasing resistance to cancer therapy. Cancer therapy resistance is caused by adaptations that favor survival within cancer cells and their environment, termed the tumor microenvironment. Intracellular adaptations include the overexpression of resistance-linked genes, such as the inhibitor of apoptosis (IAP) family of proteins and overall resistance to cell death. Adaptations in the tumor microenvironment …
Modulation Of T Lymphocytes By Tumor-Released Survivin, Jessica Marie Jutzy
Modulation Of T Lymphocytes By Tumor-Released Survivin, Jessica Marie Jutzy
Loma Linda University Electronic Theses, Dissertations & Projects
The tumor microenvironment is an area of intense interaction between normal and malignant cells. Factors and cell types within this environment can play a crucial role in the progression or regression of the tumor. Of primary interest are tumor-infiltrating T lymphocytes, which have been shown to have a key role in modifying the dynamics of the tumor microenvironment to promote or prevent tumor growth. While there is much in vitro and in vivo evidence for a modification of the tumor infiltrating T cell population toward a pro-tumor environment, what induces these changes within the tumor microenvironment has remained elusive. Our …
Hpv16 E6* Induces Oxidative Stress And Dna Damage, Vonetta M. Williams
Hpv16 E6* Induces Oxidative Stress And Dna Damage, Vonetta M. Williams
Loma Linda University Electronic Theses, Dissertations & Projects
High risk types of Human Papillomavirus are the causative agents of virtually all cases of cervical cancer, 50-90% of other anogenital cancers and approximately 30% of oral and pharyngeal cancers. The high-risk types encode two viral oncogenes, E6 and E7, which work together to initiate cell transformation. The approximately 50 amino acid product of the E6* transcript is expressed during the early stages of HPV infection. In this study, we found that expression of E6* increased the level of reactive oxygen species (ROS) in both HPV+ and HPV- cells. This increased oxidative stress led to higher levels of DNA damage. …
Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Loma Linda University Electronic Theses, Dissertations & Projects
Spatial and temporal regulation of developmental gene transcription often involves regulatory sequences found in noncoding DNA separate from the gene’s promoter. Conservation of a noncoding region (CNR) across divergent species may indicate a regulatory region critical to basic morphogenesis. CNR-green fluorescent protein (GFP) constructs can demonstrate spatial and temporal CNR activity by microscopy visualization. However, characterization of regulatory modules within a CNR requires quantitative, in addition to qualitative, analysis. The study presented here describes the development and implementation of a novel application of flow cytometry in the quantitative characterization of CNRs. The technique couples fluorescent microscopy localization with flow cytometry …
Exosomal Survivin-T34a: A Novel, Potential Cancer Therapeutic, Jonathan Richard Aspe
Exosomal Survivin-T34a: A Novel, Potential Cancer Therapeutic, Jonathan Richard Aspe
Loma Linda University Electronic Theses, Dissertations & Projects
Pancreatic cancer is the fourth most common cause of cancer fatality in American men and women with a less than 5% survival rate. Currently, if diagnosed early, surgical resection remains the only viable cure. However, only 20% of pancreatic cancer patients meet these criteria. It is therefore necessary to discover new therapies or therapeutic combinations in order to impact significantly this deadly disease. The anti-metabolite agent Gemcitabine is currently being employed to treat pancreatic cancer. While Gem-citabine has shown significant benefit in clinical applications, its ability to more than modestly impact pancreatic cancer is limited. It has been speculated that …
Effect Of Maternal Hypoxia On Fetal Development: Programming Of Hie Phenotype In Neonatal Rat Brain, Pablo J. Gonzalez-Rodriguez
Effect Of Maternal Hypoxia On Fetal Development: Programming Of Hie Phenotype In Neonatal Rat Brain, Pablo J. Gonzalez-Rodriguez
Loma Linda University Electronic Theses, Dissertations & Projects
Developmental programming of health and disease correlates the adverse intrauterine environment and the risk of disease later in life as cardiovascular disease, metabolic diseases, renal dysfunction or neurobehavioral disorders among others. Maternal hypoxia is one of the most common intrauterine growth restricted environment during pregnancy. Perinatal hypoxic-ischemic encephalopathy is an important cause of brain injury in the newborn due to a systematic asphyxia. Our study aims to test the hypothesis that maternal hypoxia affect the organogenesis of the kidney by affecting the kidney structure and focusing on the roles of AT1R and AT2R, while the brain development will also be …
Survivin: Regulation By Yy1 And Role In Pancreatic Cancer Combination Therapy, Nicholas R. Galloway
Survivin: Regulation By Yy1 And Role In Pancreatic Cancer Combination Therapy, Nicholas R. Galloway
Loma Linda University Electronic Theses, Dissertations & Projects
Despite significant clinical and basic science advancements, cancer remains a devastating disease that affects people of all ages, races, and background. Survivin, the fourth most common transcript found in cancer cells, is a protein that is thought to be involved in the enhanced proliferation, survival, and metastasis of cancer cells. Therefore understanding how this gene is regulated is potentially of vital importance to improving cancer management and therapy. Our work has identified a novel transcriptional regulator of survivin called Yin Yang 1 (YY1). YY1 is a transcription factor that has been observed to activate some gene promoters and repress others, …
Expression And Function Of Ca2+-Activated K+ Channels In Uterine Arteries, Ronghui Zhu
Expression And Function Of Ca2+-Activated K+ Channels In Uterine Arteries, Ronghui Zhu
Loma Linda University Electronic Theses, Dissertations & Projects
Chronic hypoxia during pregnancy is one of the most common insults to the maternal cardiovascular system and fetal development, and is associated with increased uterine vascular tone and heightened risk of preeclampsia and fetal intrauterine growth restriction (IUGR). The present study tested the hypothesis that calcium-activated potassium (KCa) channels play an essential role in uterine vascular adaptation to pregnancy, which is inhibited by chronic hypoxia during gestation. Uterine arteries (UAs) were isolated from nonpregnant ewes (NPUAs) and near-term pregnant ewes (PUAs) that had been maintained at sea level (~300 m) or exposed to high altitude (3,801 m) for 110 days. …
Ricin B Chain-Insulin Fusion Protein Immunomodulation Of Type 1 Diabetes, James Edward Carter Iii
Ricin B Chain-Insulin Fusion Protein Immunomodulation Of Type 1 Diabetes, James Edward Carter Iii
Loma Linda University Electronic Theses, Dissertations & Projects
Type 1 diabetes mellitus (T1D) is a debilitating chronic inflammatory disease of the insulin-producing pancreatic islet β-cells that results from a combination of genetic and environmental factors. Attempts to suppress Th1-mediated autoimmune diseases such as T1D by mucosal delivery of autoantigens for immunotolerization have yielded only partial success. Attainment of satisfactory levels of sustained immunological tolerance remains to be accomplished. To restore self-tolerance requires delivery of sufficient amounts of autoantigen to stimulate regulatory T helper cells that function to survey the gut and induce tolerance to consumed antigens such as food. Oral delivery of autoantigens has previously been shown to …
Fetal Cocaine Exposure Causes Epigenetic Changes In The Rat Heart, Kurt D. Meyer
Fetal Cocaine Exposure Causes Epigenetic Changes In The Rat Heart, Kurt D. Meyer
Loma Linda University Electronic Theses, Dissertations & Projects
Cocaine abuse continues to be prevalent in the United States and other industrialized nations, in addition to the negative health effects that cocaine abuse has on the user, a mother who uses cocaine while pregnant also exposes the developing fetus to cocaine. Although there have been many studies of the effects of cocaine on the adult heart, studies of cocaine on the fetal heart and its potential delayed pathophysiological effects on cardiac function in adult offspring are extremely limited. The studies of the present project sought to enhance the understanding of the effect of cocaine exposure on the fetal heart …
Effects Of Spaceflight On Phagocytic Function And Immune-Neural Interaction, Farnaz P. Baqai
Effects Of Spaceflight On Phagocytic Function And Immune-Neural Interaction, Farnaz P. Baqai
Loma Linda University Electronic Theses, Dissertations & Projects
In spaceflight, astronauts will be exposed to several environmental risk factors that may lead to disturbances in homeostasis maintained by the central nervous system (CNS) and immune system. Low Earth orbit (LEO) spaceflight studies have shown that microgravity changes virtually all immune parameters that have been evaluated. Similarly, ground based studies have shown that low-dose radiation can also significantly impact immune function. Finally, as most spacecrafts are ecologically and environmentally closed systems, astronauts may be at increased risk for exposure to aerosolized infectious agents. The hypothesis of this study is: Exposure to spaceflight environment (microgravity and radiation) can influence immune …
Mnte-2-Pyp And Radiation In A Prostate Cancer Model: Implications For Radiotherapy, Adeola Y. Makinde
Mnte-2-Pyp And Radiation In A Prostate Cancer Model: Implications For Radiotherapy, Adeola Y. Makinde
Loma Linda University Electronic Theses, Dissertations & Projects
A major limitation of successful radiation therapy in cancer treatment is the increase in normal tissue damage as higher doses are used to achieve greater tumor destruction. Radiation dose optimization in cancer therapy requires achieving maximum tumor destruction with minimal damage to normal tissue Antioxidants have been shown to protect normal tissues against radiation damage, as radiation-induced tissue damage results predominantly from reactive oxygen species that directly damage cellular components. However, for effective use as normal tissue radioprotectants in radiotherapy, these antioxidants must not protect the tumors. Mn (III) tetrakis (N-ethylpyridinium-2-yl) porphyrin (MnTE-2-PyP) is a metalloporphyrin antioxidant that has been …
Localization And Expression Of The Aer Receptor In Escherichia Coli, Daniel Salcedo
Localization And Expression Of The Aer Receptor In Escherichia Coli, Daniel Salcedo
Loma Linda University Electronic Theses, Dissertations & Projects
In Escherichia coli, chemotactic movement towards an energetically-favorable environment is mediated by five transmembrane chemoreceptors. These sensory proteins respond to numerous environmental signals, including amino acids, sugars, and pH. The aerotaxis receptor, Aer, is an intracellular sensor that responds to oxygen concentration and other parameters affecting cellular energy levels. Bacterial chemotaxis receptors form dimeric signaling units that organize into tetramers and hexamers and form large clusters at the cell poles. A cluster arrangement could increase receptor sensitivity by lateral communication between dimers, thus amplifying the signal from one dimer. We have shown that Aer forms dimers, trimer of dimers, …
Role Of No In The Regulation Of Cortisol Synthesis In Long-Term Hypoxic Sheep Fetal Adrenal, Tshepo Rothi Monau
Role Of No In The Regulation Of Cortisol Synthesis In Long-Term Hypoxic Sheep Fetal Adrenal, Tshepo Rothi Monau
Loma Linda University Electronic Theses, Dissertations & Projects
Our laboratory has demonstrated that sheep fetuses adapt to long-term hypoxia (LTH) by maintaining normal level of basal plasma cortisol despite increased adrenocorticotropic hormone (ACTH). But the mechanisms involved are not fully understood. Studies have shown that nitric oxide (NO) inhibits synthesis of steroid hormones in a variety of tissues, and in different animal species. Studies also showed that hypoxia increases both expression and activity levels of the nitric oxide (NO) generating enzyme, nitric oxide synthase (NOS), in certain tissues. We have here investigated the expression level and localization of NOS isoforms in sheep fetal adrenals. We also investigated the …
Hypochlorous Acid Mediated Damage Of Chromatin, Joseph Insugn Kang
Hypochlorous Acid Mediated Damage Of Chromatin, Joseph Insugn Kang
Loma Linda University Electronic Theses, Dissertations & Projects
Hypochlorous acid (HOCl), the primary product of activated neutrophils at sites of inflammation, can damage both DNA and associated histone proteins. HOCl damage to cytosine bases in DNA generates the chlorination damage products 5-chlorocytosine (ClC) and 5-chlorouracil (ClU) as well as the oxidation damage products 5- hydroxycytosine (HOC) and 5-hydroxyuracil (HOU). Histone damage by HOCl can be measured by quantitation of 3-chlorotyrosine and 3,5-dichlorotyrosine, stable and unique markers of protein damage caused by HOCl. The studies presented here investigate how and where these damage products occur and what implications they might have in the correlation between chronic inflammation and carcinogenesis. …
Biochemical Characteristics Of Inconsistent Free T₄ Assays, Kristofer S. Fritz
Biochemical Characteristics Of Inconsistent Free T₄ Assays, Kristofer S. Fritz
Loma Linda University Electronic Theses, Dissertations & Projects
Serum levels of free thyroxine (T4) are helpful in the clinical evaluation of patients who are suspected of having thyroid disease, since free T4 (unbound) is known to closely relate to hyper, eu and hypothyroidism. There are documented inconsistencies among commercially available direct free thyroxine immunoassays. The biochemical basis for these inconsistencies is not understood and has not been characterized. Direct free T4 measurements have been linked to both T4-binding serum protein concentrations and protein-bound T4 concentrations. A free T4 reference method using direct equilibrium dialysis radioimmunoassay has been well characterized. New …
Iron Dysregulation And Inflammation In Alzheimer’S Disease, Shino D. Magaki
Iron Dysregulation And Inflammation In Alzheimer’S Disease, Shino D. Magaki
Loma Linda University Electronic Theses, Dissertations & Projects
Alzheimer’s disease (AD) is the most common form of senile dementia in the US and worldwide but the causes of its pathogenesis are currently unknown. In this study, we examined two processes that have been implicated in the early stages of AD and other forms of neurodegeneration, iron dysregulation and inflammation, both of which can promote the increased production of amyloid precursor protein (APP). We have measured different pools of brain iron in transgenic iron regulatory protein 2 knockout (IRP2-/-) mice in the early stages of neurodegeneration and in affected brain regions from AD patients at different stages of the …
Radiation-Induced Increases In Pkc Modulate Integrin Expression And Contribute To Fibrotic Changes, Pinal Rushikesh Pandya
Radiation-Induced Increases In Pkc Modulate Integrin Expression And Contribute To Fibrotic Changes, Pinal Rushikesh Pandya
Loma Linda University Electronic Theses, Dissertations & Projects
The sequences of events in fibrosis are similar to those in wound healing; however, the normal termination and resolution stages do not take place. The initial cellular response following ionizing radiation involves accumulation of the ECM (extracellular matrix) including collagen, fibronectin and the interaction of many growth factors (cytokines) with their receptors. There are a number of unanswered questions regarding many aspects of radiation-induced fibrosis, including the initial triggers and physical changes that initiate the process. We have confirmed that elevated pKC and other cellular changes following radiation are similar to those found at sites of inflammation. This information leads …