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Full-Text Articles in Life Sciences
Architecture Of The Potyviruses, Michele Mcdonald, Amy Kendall, Wen Bian, Ian Mccullough, Elizabeth Lio, Wendy Havens, Said Ghabrial, Gerald Stubbs
Architecture Of The Potyviruses, Michele Mcdonald, Amy Kendall, Wen Bian, Ian Mccullough, Elizabeth Lio, Wendy Havens, Said Ghabrial, Gerald Stubbs
Ian McCullough
X-ray fiber diffraction data were obtained and helical pitch and symmetry were determined for seven members of the family Potyviridae, including representatives from the genera Potyvirus, Rymovirus, and Tritimovirus. The diffraction patterns are similar, as expected. There are, however, significant variations in the symmetries, as previously found among the flexible potexviruses, but not among the rigid tobamoviruses. Wheat streak mosaic virus, the only member of the genus Tritimovirus examined, displayed the largest deviations in diffraction data and helical parameters from the other viruses in the group.
Flexible Filamentous Virus Structures From Fiber Diffraction, Gerald Stubbs, Amy Kendall, Michele Mcdonald, Wen Bian, Timothy Bowles, Sarah Baumgarten, Ian Mccullough, Jian Shi, Phoebe Stewart, Esther Bullitt, David Gore, Said Ghabrial
Flexible Filamentous Virus Structures From Fiber Diffraction, Gerald Stubbs, Amy Kendall, Michele Mcdonald, Wen Bian, Timothy Bowles, Sarah Baumgarten, Ian Mccullough, Jian Shi, Phoebe Stewart, Esther Bullitt, David Gore, Said Ghabrial
Ian McCullough
Fiber diffraction data have been obtained from Narcissus mosaic virus, a potexvirus from the family Flexiviridae, and soybean mosaic virus (SMV), a potyvirus from the family Potyviridae. Analysis of the data in conjunction with cryo-electron microscopy data allowed us to determine the symmetry of the viruses and to make reconstructions of SMV at 19 Å resolution and of another potexvirus, papaya mosaic virus, at 18 Å resolution. These data include the first well-ordered data ever obtained for the potyviruses and the best-ordered data from the potexviruses, and offer the promise of eventual high resolution structure determinations.
Radial Density Distribution And Symmetry Of A Potexvirus, Narcissus Mosaic Virus, Amy Kendall, Wen Bian, Justin Junn, Ian Mccullough, David Gore, Gerald Stubbs
Radial Density Distribution And Symmetry Of A Potexvirus, Narcissus Mosaic Virus, Amy Kendall, Wen Bian, Justin Junn, Ian Mccullough, David Gore, Gerald Stubbs
Ian McCullough
Narcissus mosaic virus is a Potexvirus, a member of the Flexiviridae family of filamentous plant viruses. Fiber diffraction patterns from oriented sols of narcissus mosaic virus have been used to determine the symmetry and structural parameters of the viral helix. The virions have a radius of 55 ± 5 Å. The viral helix has a pitch of 34.45 ± 0.5 Å, with 7.8 subunits per turn of the helix. We conclude that all members of the Potexvirus genus have close to 8 subunits per helical turn.
Wcen: A Computer Program For Initial Processing Of Fiber Diffraction Patterns, Wen Bian, Hong Wang, Ian Mccullough, Gerald Stubbs
Wcen: A Computer Program For Initial Processing Of Fiber Diffraction Patterns, Wen Bian, Hong Wang, Ian Mccullough, Gerald Stubbs
Ian McCullough
Processing of fiber diffraction patterns is generally more difficult than for single-crystal patterns, and requires different algorithms and software. The program WCEN has been developed to determine experimental and specimen parameters and to convert diffraction data from detector to reciprocal space, and offers a variety of input and output formats, running under Mac OS X and Linux. The program is described and examples from oriented sols of filamentous plant viruses, illustrating different strategies for parameter determination and refinement, are given.