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Characterization Of The Interaction Between Bone Sialoprotein And Type I Collagen, Rose Yee
Characterization Of The Interaction Between Bone Sialoprotein And Type I Collagen, Rose Yee
Electronic Thesis and Dissertation Repository
The mechanism of biomineralization is unknown. In bone, it has been proposed that an acidic phosphoprotein of the extracellular matrix (ECM) is important in the nucleation of hydroxyapatite (HA). The mineralized tissue protein, bone sialoprotein (BSP), has been shown to be a potent nucleator of HA and this activity is increased upon binding to type I collagen. The collagen-binding domain of BSP has been determined to be a highly conserved region (spanning residues 18-45); however, the area of collagen involved in this interaction is unknown.
In this study, a chemical cross-linking method was initially utilized to map the BSP-binding region …