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Biochemistry, Biophysics, and Structural Biology
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Regulation Of Gene Expression By Rna Binding Proteins And Micrornas, Kyle Cottrell
Regulation Of Gene Expression By Rna Binding Proteins And Micrornas, Kyle Cottrell
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Regulation of gene expression is essential to life. Post-transcriptional regulation of gene expression is a complex process with many inputs that lead to changes in localization, translation and stability of mRNAs. The translation and stability of many mRNAs is regulated by cis-elements, such as mRNA-structure or codon optimality; and by trans-acting factors such as RBPs and miRNAs. Here I report on the complex interactions between RBPs, miRNAs and characteristics of their target mRNAs in respect to effects on translation and RNA stability.
Using a reporter based approach we studied modulation of microRNA-mediated repression by various mRNA characteristics. We observed the …