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Full-Text Articles in Life Sciences
Mechanism Of Iron Transport In Mycelia Sterilia Ep-76, Jonathan P. Adjimani
Mechanism Of Iron Transport In Mycelia Sterilia Ep-76, Jonathan P. Adjimani
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The cyclic trihydroxamic acid, N, N', N' '-triacetylfusarinine C, produced by Mycelia sterilia EP-76, is shown to be a ferric ionophore for this organism. The association constant for ferric-N, N', N' '-triacetylfusarinine C complex was determined to be log K=32.5. Other iron chelating agents, such as rhodotorulic acid, citric acid, or the monomeric subunit of triacetylfusarinine C, N-acetyl- fusarinine, delivered iron to the cells by an indirect mechanism involving iron exchange into triacetylfusarinine C. In vitro ferric ion exchange was found to be rapid with triacetylfusarinine C. Gallium uptake rates comparable to those of iron were observed with the chelating …
An Improved Method For Transferring Nucleic Acids To Nylon Membranes, Bruce D. Parker
An Improved Method For Transferring Nucleic Acids To Nylon Membranes, Bruce D. Parker
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Methods currently in use for the separation and identification of specific segments of nucleic acids involve long transfer periods or elaborate apparatuses and result in the production of a single blot. Contamination by organisms or enzymes is always a factor to be dealt with. An improved method for transferring nucleic acids from acrylamide or agarose gels for use in hybridization has been developed. This method uses NaOH as the blotting medium to improve the rate and efficiency of transfer to nylon membranes. As many as six blots can be obtained within one hour using this method. This method is effective …