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Development Of 3d Bioartificial Human Tissue Models Of Periprosthetic Shoulder Joint Infection, Tony B. Huang
Development Of 3d Bioartificial Human Tissue Models Of Periprosthetic Shoulder Joint Infection, Tony B. Huang
Electronic Thesis and Dissertation Repository
Periprosthetic joint infection (PJI) is a devastating and costly post-surgical complication that is not well understood due to the scarcity of physiologically representative experimental models. This thesis outlines the development of two 3D bioartificial human tissue models designed to study the cellular and biochemical interactions between primary fibroblasts from the shoulder capsule (SC) and infectious microorganisms. Using the Fibroblast-Bacteria Co-culture in 3D Collagen model, we demonstrated a global gene repression of metabolic and homeostatic processes in SC fibroblasts following 48 hours of co-culture with Cutibacterium acnes – the most common microbial cause of PJI in the shoulder. These cellular changes …