Life Sciences Commons^™

Glucose Mediated Effects On Glucose-6-Phosphate Dehydrogenase Expression, Daryl Arkwright

Masters Theses

Circulating high glucose levels are believed to cause oxidative stress which is implicated in a number of pathological conditions such as cancer, aging, and diabetes. Oxidative stress regulates the expression of glucose-6-phosphate dehydrogenase (G6PDH), the key enzyme in the pentose phosphate pathway which provides reducing equivalents in the form of NADPH. These reducing equivalents are also required by fatty acid synthase (FAS), the enzyme which catalyzes fatty acid biosynthesis. We examined whether glucose, a known inducer of oxidative stress, could regulate either G6PDH or FAS activity in primary rat hepatocytes. We tested the effects of glucose alone or in combination …

Molecular Staging Of Colorectal Cancer: K-Ras Mutation Analysis Of Lymph Nodes Upstages Dukes’ B2 Patients, Jennifer S. Thebo

Dissertations

The successful treatment of colorectal cancer depends upon the ability of staging systems to identify patients who are at risk for recurrence. The currently used systems fail in a significant number of patients. Multiple attempts have been made to improve upon these systems in order to provide better treatment for at-risk patients.

A variety of oncogenes and tumor suppressor genes have been implicated in colorectal cancer. Mutations in one of these, K-ras, occur in 40-60% of colorectal carcinomas. However, the relationship between specific mutations and clinical outcome is unclear.

The purpose of this study was to determine whether or not …