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Full-Text Articles in Life Sciences
Study Of The Baie Gene In Bile Acid 7Α-Dehydroxylating Bacteria, Yasemin Kaya
Study Of The Baie Gene In Bile Acid 7Α-Dehydroxylating Bacteria, Yasemin Kaya
Masters Theses & Specialist Projects
Intestinal bile acid 7α-dehydroxylating bacteria have recently been implicated in cholesterol allstone disease. Eubacterium sp. V.P.I. 12708, a bile acid 7α-dehydroxylating bacterium, contains multiple bile acid inducible (bai) genes which encode the enzymes responsible for bile acid 7a-dehydroxylation. The baiE gene encodes a bile acid dehydratase activity in Eubacterium sp.V.P.I. 12708. Using the polymerase chain reaction assay we determined the presence or absence of baiE-like genes in five clostridial bile-acid 7α-dehydroxylating strains: Clostridium sp. TO-931, Clostridium sp. HD-17, Clostridium sp. TN-271, Clostridium bifermentans 1-55, and Clostridium sordellii ATCC 9714. Results from all the strains tested showed amplification at the predicted …
The Establishment Of Standard Methods And Experimental Procedures For The Prototypical Icp/Tof-Ms Using An Icp-Aes, Joseph Riley
The Establishment Of Standard Methods And Experimental Procedures For The Prototypical Icp/Tof-Ms Using An Icp-Aes, Joseph Riley
Mahurin Honors College Capstone Experience/Thesis Projects
The ICP-AES was used to develop a standard test method for the preparation of samples for analysis purposes, a method that could also be transposed onto the new prototypical ICP/TOF-MS. Whereas both of these instruments are analytical spectroscopic instruments and use the ICP's plasma as an energy source, the ICP/TOF-MS also has a mass spectrometer attached to it. This addition gives the instrument better reliability, accuracy, and precision in analysis as well as faster analysis time. Since the ICP-AES is a precursor of the ICP/TOF-MS, it can be used to develop a standard development method. The experiment involved using a …