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Investigation Of Proline Utilization A: Kinetic Analysis Of Substrate Channel-Blocking Mutants And Creation Of A Trifunctional Chimera Enzyme, Benjamin W. Arentson
Investigation Of Proline Utilization A: Kinetic Analysis Of Substrate Channel-Blocking Mutants And Creation Of A Trifunctional Chimera Enzyme, Benjamin W. Arentson
Department of Biochemistry: Dissertations, Theses, and Student Research
Proline metabolism is known to be involved in many cellular processes such as cell signaling, cellular redox balance, and cell survival. One of the enzymes involved in proline catabolism, proline utilization A, plays a role in oxidizing proline to glutamate in a two-step oxidation pathway involving enzymes proline dehydrogenase (PRODH) and Δ1-pyrroline-5-carboxylate dehydrogenase (P5CDH).
Intermediate P5C/GSA has been shown to use an intramolecular channel to move from the PRODH active site to the P5CDH active site in a phenomenon called substrate channeling. In this work, one of the main objectives was to learn more about the channel usage. …