Open Access. Powered by Scholars. Published by Universities.®
- File Type
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Flexible Filamentous Virus Structures From Fiber Diffraction, Gerald Stubbs, Amy Kendall, Michele Mcdonald, Wen Bian, Timothy Bowles, Sarah Baumgarten, Ian Mccullough, Jian Shi, Phoebe Stewart, Esther Bullitt, David Gore, Said Ghabrial
Flexible Filamentous Virus Structures From Fiber Diffraction, Gerald Stubbs, Amy Kendall, Michele Mcdonald, Wen Bian, Timothy Bowles, Sarah Baumgarten, Ian Mccullough, Jian Shi, Phoebe Stewart, Esther Bullitt, David Gore, Said Ghabrial
Ian McCullough
Fiber diffraction data have been obtained from Narcissus mosaic virus, a potexvirus from the family Flexiviridae, and soybean mosaic virus (SMV), a potyvirus from the family Potyviridae. Analysis of the data in conjunction with cryo-electron microscopy data allowed us to determine the symmetry of the viruses and to make reconstructions of SMV at 19 Å resolution and of another potexvirus, papaya mosaic virus, at 18 Å resolution. These data include the first well-ordered data ever obtained for the potyviruses and the best-ordered data from the potexviruses, and offer the promise of eventual high resolution structure determinations.
Radial Density Distribution And Symmetry Of A Potexvirus, Narcissus Mosaic Virus, Amy Kendall, Wen Bian, Justin Junn, Ian Mccullough, David Gore, Gerald Stubbs
Radial Density Distribution And Symmetry Of A Potexvirus, Narcissus Mosaic Virus, Amy Kendall, Wen Bian, Justin Junn, Ian Mccullough, David Gore, Gerald Stubbs
Ian McCullough
Narcissus mosaic virus is a Potexvirus, a member of the Flexiviridae family of filamentous plant viruses. Fiber diffraction patterns from oriented sols of narcissus mosaic virus have been used to determine the symmetry and structural parameters of the viral helix. The virions have a radius of 55 ± 5 Å. The viral helix has a pitch of 34.45 ± 0.5 Å, with 7.8 subunits per turn of the helix. We conclude that all members of the Potexvirus genus have close to 8 subunits per helical turn.