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The B Subunits In The Peripheral Stalk Of F1f0 Atp Synthase Preferentially Adopt An Offset Relationship, Shane Claggett, Mac O'Neil Plancher, Stanley Dunn, Brian Cain
The B Subunits In The Peripheral Stalk Of F1f0 Atp Synthase Preferentially Adopt An Offset Relationship, Shane Claggett, Mac O'Neil Plancher, Stanley Dunn, Brian Cain
Stanley D Dunn
The peripheral stalk of F1F0 ATP synthase is essential for the binding of F1 to FO and for proper transfer of energy between the two sectors of the enzyme. The peripheral stalk of Escherichia coli is composed of a dimer of identical b subunits. In contrast, photosynthetic organisms express two b-like genes that form a heterodimeric peripheral stalk. Previously we generated chimeric peripheral stalks in which a portion of the tether and dimerization domains of the E. coli b subunits were replaced with homologous sequences from the b and b' subunits of Thermosynechococcus elongatus (Claggett, S. B., Grabar, T. B., …
Functionally Compensating Coevolving Positions Are Neither Homoplasic Nor Conserved In Clades, Gregory Gloor, Gaurav Tyagi, Dana Abrassart, Andrew Kingston, Andrew Fernandes, Stanley Dunn, Christopher Brandl
Functionally Compensating Coevolving Positions Are Neither Homoplasic Nor Conserved In Clades, Gregory Gloor, Gaurav Tyagi, Dana Abrassart, Andrew Kingston, Andrew Fernandes, Stanley Dunn, Christopher Brandl
Stanley D Dunn
We demonstrated that a pair of positions in phosphoglycerate kinase that score highly by three nonparametric covariation measures are important for function even though the positions can be occupied by aliphatic, aromatic, or charged residues. Examination of these pairs suggested that the majority of the covariation scores could be explained by within-clade conservation. However, an analysis of diversity showed that the conservation within clades of covarying pairs was indistinguishable from pairs of positions that do not covary, thus ruling out both clade conservation and extensive homoplasy as means to identify covarying positions. Mutagenesis showed that the residues in the covarying …