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Full-Text Articles in Life Sciences
Antagonistic Roles Of Grk2 And Grk5 In Cardiac Aldosterone Signaling Reveal Grk5-Mediated Cardioprotection Via Mineralocorticoid Receptor Inhibition, Jennifer Maning, Katie Anne Mccrink, Celina M. Pollard, Victoria Desimine, Jennifer Ghandour, Arianna Perez, Natalie Cora, Krysten E. Ferraino, Barbara Parker, Ava Brill, Beatrix Aukszi, Anastasios Lymperopoulos
Antagonistic Roles Of Grk2 And Grk5 In Cardiac Aldosterone Signaling Reveal Grk5-Mediated Cardioprotection Via Mineralocorticoid Receptor Inhibition, Jennifer Maning, Katie Anne Mccrink, Celina M. Pollard, Victoria Desimine, Jennifer Ghandour, Arianna Perez, Natalie Cora, Krysten E. Ferraino, Barbara Parker, Ava Brill, Beatrix Aukszi, Anastasios Lymperopoulos
Chemistry and Physics Faculty Articles
Aldosterone (Aldo), when overproduced, is a cardiotoxic hormone underlying heart failure and hypertension. Aldo exerts damaging effects via the mineralocorticoid receptor (MR) but also activates the antiapoptotic G protein-coupled estrogen receptor (GPER) in the heart. G protein-coupled receptor (GPCR)-kinase (GRK)-2 and -5 are the most abundant cardiac GRKs and phosphorylate GPCRs as well as non-GPCR substrates. Herein, we investigated whether they phosphorylate and regulate cardiac MR and GPER. To this end, we used the cardiomyocyte cell line H9c2 and adult rat ventricular myocytes (ARVMs), in which we manipulated GRK5 protein levels via clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and …