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Compartmentation Of Glutamate Metabolism Observed With Glucose / D. D. Clarke, E. Dicker & E.J. Ronan, Chem. Dept. Fordham University, Bronx, N.Y. 10458, U.S.A., Donald Dudley Clarke Phd, E. Dicker, E. J. Ronan
Compartmentation Of Glutamate Metabolism Observed With Glucose / D. D. Clarke, E. Dicker & E.J. Ronan, Chem. Dept. Fordham University, Bronx, N.Y. 10458, U.S.A., Donald Dudley Clarke Phd, E. Dicker, E. J. Ronan
Chemistry Faculty Publications
Compartmentation of glutamate metabolism in the central nervous system has been demonstrated in a number of laboratories using a variety of labeled precursors. The labeling of glutamine is much more rapid than would be expected if the total pool of glutamate in brain is the precursor of glutamine. An obvious suggestion for the origin of this compartmentation is that it reflects glial vs. neuronal relationships. Some evidence in support of this idea has been obtained by Rose using separated neuronal vs. neuropil preparations (J. Neurochem. 17, 809 (1970)). Until recently orain was the only tissue which, after the injection of …
Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl
Labeling Of Glutamate, Glutamine, And Aspartate In Brain From C14 Aspartate And Acetate / W. J. Ncklas D. D. Clarke And S. Berl Chemistry Dept., Fordham University And Col. Of Physicians And Surgeons, Columbia University, William J. Nicklas, Donald Dudley Clarke Phd, Soll Berl
Chemistry Faculty Publications
Guinea pig brain cortex slices incubated in media containing U-C14 aspartic acid form glutamine of higher specific activity than glutamic acid (Fed. Proc. -25, 713 (1966) ). Glutamic acid and glutamine from such experiments were enzymatically decarboxylated and the o/o of label in C-1 ranged from 37 to 42o/o. These values exceed the maximum predicted from the known operation of the citric acid cycle viz. 33o/o. When l-C14 acetate was injected into mice or incubated with brain slices the aspartate isolated from brain had more than 50o/o of the label in C-4. From the known operation of the citric acid …