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Oxidative Metabolism Of 4-Aminobutyrate By Rat Brain Mitochondria: Inhibition By Branched-Chain Fatty Acid / J. Cunningham, D. D. Clarke, And W.J. Nicklas, J. Cunningham, Donald Dudley Clarke Phd, William J. Nicklas
Oxidative Metabolism Of 4-Aminobutyrate By Rat Brain Mitochondria: Inhibition By Branched-Chain Fatty Acid / J. Cunningham, D. D. Clarke, And W.J. Nicklas, J. Cunningham, Donald Dudley Clarke Phd, William J. Nicklas
Chemistry Faculty Publications
The oxidation of 4-aminobutyric acid (GABA) by nonsynaptosomal mitochondria isolated from rat forebrain and the inhibition of this metabolism by the branched-chain fatty acids 2-methyl-2-ethyl caproate (MEC) and 2,2- dimethyl valerate (DMV) were studied. The rate of GABA oxidation, as measured by 02 uptake, was determined in medium containing either 5 or 100 mM-[K+]. The apparent Km for GABA was 1.16 ± 0.19 mM and the Vmax in state 3 was 23.8 + 5.5 ng-atoms O2 ·min-1.mg protein-1 in 5 mM-[K+]. In a medium with 100 mM-[K+] the apparent K111 was 1.11 + 0.17 mM and V max was 47.4 …