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The Pyruvate, Orthophosphate Dikinase Regulatory Proteins Of Arabidopsis Possess A Novel, Unprecedented Ser/Thr Protein Kinase Primary Structure, Chris J. Chastain, Wenxin Xu, Kate Parsley, Gautam Sarath, Jullian Hebberd, Raymond Chollet
The Pyruvate, Orthophosphate Dikinase Regulatory Proteins Of Arabidopsis Possess A Novel, Unprecedented Ser/Thr Protein Kinase Primary Structure, Chris J. Chastain, Wenxin Xu, Kate Parsley, Gautam Sarath, Jullian Hebberd, Raymond Chollet
Department of Biochemistry: Faculty Publications
Pyruvate, orthophosphate dikinase (PPDK) is a ubiquitous, low-abundance metabolic enzyme of undetermined function in C3 plants. Its activity in C3 chloroplasts is light-regulated via reversible phosphorylation of an active-site Thr residue by the PPDK regulatory protein (RP), a most unusual bifunctional protein kinase (PK)/ protein phosphatase (PP). In this paper we document the molecular cloning and functional analysis of the two unique C3 RPs in Arabidopsis thaliana. The first of these, AtRP1, encodes a typical chloroplast-targeted, bifunctional C4-like RP. The second RP gene, AtRP2, encodes a monofunctional polypeptide that possesses in vitro RP-like PK activity but lacks PP activity, …