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Full-Text Articles in Life Sciences
World-Class Research: Biomedical Science Beyond Experimentation
World-Class Research: Biomedical Science Beyond Experimentation
Colby Magazine
Faculty and relationships with Maine’s big-three research labs propel Colby’s opportunities in biomedical science
Could childhood nutrition set late-life brain function?
Hitting the lab running
Ahmad wins grant for study of neurodegenerative disease
An Investigation Of Bag3 Knockdown And Its Effects On The Cytotoxicity Of Laromustine, Colin Sheehan
An Investigation Of Bag3 Knockdown And Its Effects On The Cytotoxicity Of Laromustine, Colin Sheehan
Honors Theses
Laromustine is a sulfonylhydrazine anticancer prodrug whose main cytotoxic arises from its ability to interfere with DNA replication of dividing cells. Multiple studies have suggested that Laromustine induces a form of cell death known as apoptosis. In a previous study investigating the mechanism of apoptosis, bcl2-associated athanogene 3 (BAG3) demonstrated significant upregulation in the presence of Laromustine. Given its anticancer ability, we selected BAG3 as a target for further investigation. BAG3 knockdown through transient shRNA transfections was performed in U138 glioblastoma multiforme cells and verified using qRT-PCR analysis. Finally, cell death assays were used to assess Laromustine’s cytotoxic effect on …