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Articles 1 - 22 of 22
Full-Text Articles in Life Sciences
The Effects Of Induced Polycystic Ovary Syndrome In Nag-1 Transgenic Mice, Nicholas Werner
The Effects Of Induced Polycystic Ovary Syndrome In Nag-1 Transgenic Mice, Nicholas Werner
All Master's Theses
Polycystic ovary syndrome (PCOS) is the leading cause of infertility among women in the US and the most common endocrine disorder among women. PCOS is characterized by cystic ovaries, hyperandrogenism (heightened levels of male sex hormones), altered menstrual cycles and various metabolic dysfunctions. The metabolic symptoms associated with PCOS are difficult to treat, as they are a result of hormonal imbalances, rather than diet. The human Non-Steroidal Anti-Inflammatory Drug Activated Gene (NAG-1) been shown to prevent diet-induced metabolic disorders and weight gain in mice. We hypothesized that the expression of NAG-1 may also prevent hormonal-induced metabolic disorders. To test this …
Acoustic Analysis Of Nomascus Gibbon Songs As A Potential Measure Of Current Health Status, Caroline Rowley
Acoustic Analysis Of Nomascus Gibbon Songs As A Potential Measure Of Current Health Status, Caroline Rowley
All Master's Theses
Post-release monitoring is vital to the rehabilitation process. Gibbons offer a unique challenge, as they are notoriously difficult to follow through the forest, but their duets offer a non-invasive window into their lives. The aim of this project is to create a method that will evaluate the health of rehabilitated and released gibbons through acoustic analyses. Gibbon duets are in part genetically determined, but learning may play a role in song development. Additionally, songs may indicate resource holding potential, and are vital to acquiring mates and producing offspring. Captive-raised gibbons are often denied the experience of co-singing with their parents, …
Investigating Potential Multi-Protein Complexes Involving Emx2, Nicole Enger
Investigating Potential Multi-Protein Complexes Involving Emx2, Nicole Enger
All Master's Theses
The neocortex of the mammalian brain is allocated into specialized areas during embryonic development. In mice, as well as humans, the neocortex develops four primary areas: somatosensory, auditory, visual, and motor. In the adult neocortex, the boundaries between these four areas are marked by differences in cellular architecture and gene expression patterns. However, these physical boundaries are not evident during embryonic development of this structure. Rather, the neocortex initially appears uniform across its expanse, with one exception: a handful of proteins that regulate neocortical arealization are generated in gradients across the expanse of the neocortex and control the size of …
Measuring Trace Element Concentrations In Artiodactyl Cannonbones Using Portable X-Ray Fluorescence, Joshua L. Henderson
Measuring Trace Element Concentrations In Artiodactyl Cannonbones Using Portable X-Ray Fluorescence, Joshua L. Henderson
All Master's Theses
Artiodactyl bones are the most common faunal remains found in Washington prehistoric archaeology sites, but they are often too fragmented to accurately identify a family, genus, or species. Traditional faunal analysis can only organize unidentifiable bone fragments into size class, and chemical methods often require the destruction of bone samples. In this thesis research, I tested a new, nondestructive faunal analysis technique using portable X-ray fluorescence (pXRF) to measure trace element concentrations in comparative collection and archaeological bone samples. Using cannonbones from five different artiodactyl species, I collected trace element data from 50 comparative collection specimens and 18 archaeological specimens …
Do Elevated Levels Of Glutathione Decrease The Toxicity Of Plasticizers In Mouse Liver Cells?, Khatoon Albahrani
Do Elevated Levels Of Glutathione Decrease The Toxicity Of Plasticizers In Mouse Liver Cells?, Khatoon Albahrani
All Master's Theses
Phthalates are esters of phthalic acid that play a crucial role in the manufacturing industries for enhancing the properties of plastic materials. Di-(2-ethylhexyl) phthalate (DEHP) is one of the most common phthalates used in a variety of products including food packaging and medical equipment. Their use has caused public health concerns because of their toxic effects on reproductive and developmental processes. This study aims to investigate the response of two mouse hepatoma cell lines to the effects of DEHP and its bioactive metabolite mono (2-ethylhexyl) phthalate (MEHP). Cells were exposed to four concentrations (10, 50, 100, and 300 µM) of …
Effect Of Quantum Dots, Novel Biological Imaging Agents, In Liver Cells In Vitro, Utshaha Maharjan
Effect Of Quantum Dots, Novel Biological Imaging Agents, In Liver Cells In Vitro, Utshaha Maharjan
All Master's Theses
The use of Quantum dots (QDs) coated with polymer and functionalized with carboxylic acid groups in medical applications are explored. Their water solubility and exceptional stability in aqueous environments make them potentially useful for such applications as imaging and ligand attachments. However, there are concerns regarding the toxic effects of QDs and the minimal dose that can be used without producing any detrimental effects to organisms. In this study, QDs coated with the amphiphilic polymer coating tri-n-octylphosphine oxide and poly (maleic anhydride-alt-1-tetradecene (TOPO-PMAT)) which is functionalized with carboxylic acid groups were used to investigate their toxic effect in mouse liver …
Confirmation Of Emx2 Protein Binding Partners, Cody Gillman
Confirmation Of Emx2 Protein Binding Partners, Cody Gillman
All Master's Theses
The neocortex is a structure within mammalian brains that processes sensory input from eyes, ears, and touch receptors and mediates the conscious use of skeletal muscles. The processing of information related to each of these types of functions is localized within discrete areas of the neocortex, which are separated by sharp borders. Proper development of these functional areas is regulated during embryogenesis by several transcription factors that are expressed in distinct gradients across the progenitor layer of the neocortex, the ventricular zone. Despite the vast amount of progress that has been made in describing how these transcription factors impact the …
Efficacy Of Ultraviolet Radiation To Control Lactic Acid Bacteria In Wine Must, Brian D. Williams
Efficacy Of Ultraviolet Radiation To Control Lactic Acid Bacteria In Wine Must, Brian D. Williams
All Master's Theses
The use of shortwave ultraviolet (UVc) radiation to control lactic acid bacteria (LAB) in wine production was studied. A simulated wine sterilizer was built using a commercially sourced ultraviolet (UV) sterilizer commonly used in aquariums and ponds. After growing cultures in test tubes, samples of five different species of LAB were introduced into white grape juice adjusted for brix and pH to match that of wine must commonly found in the Yakima Valley American Viticultural Area. The mixture was then agitated and allowed time to evenly distribute the bacteria throughout the juice. The juice was sent through the …
Physiological Variation Of Garry Oak (Quercus Garryana) Seedlings To Drought Stress, Matthew A. Merz
Physiological Variation Of Garry Oak (Quercus Garryana) Seedlings To Drought Stress, Matthew A. Merz
All Master's Theses
The purpose of this study was to determine physiological differences in drought response among Garry oak (Quercus garryana Douglas ex Hook. [Fagaceae]) seedlings that grow in distinct populations in Washington state, in order to aid in restoration efforts by informing growers of the potential differences between the acorn collection sites. Acorns from six Washington populations east of the Cascades, as well as one population from Whidbey Island, were collected and grown in containers under controlled conditions. The plants were assessed with and without moderate and severe drought stress induced by withholding water. The most extreme differences in photosynthetic characteristics …
Antimicrobial And Antiinsectan Phenolic Metabolites Of Dalea Searlsiae, Gil Belofsky, Mario Aronica, Eric Foss, Jane Diamond, Felipe Santana, Jacob Darley, Patrick F. Dowd, Christina M. Coleman, Daneel Ferreira
Antimicrobial And Antiinsectan Phenolic Metabolites Of Dalea Searlsiae, Gil Belofsky, Mario Aronica, Eric Foss, Jane Diamond, Felipe Santana, Jacob Darley, Patrick F. Dowd, Christina M. Coleman, Daneel Ferreira
All Faculty Scholarship for the College of the Sciences
Continued interest in the chemistry of Dalea spp. led to investigation of Dalea searlsiae, a plant native to areas of the western United States. Methanol extractions of D. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans A–D (1–4) and known flavanones (5 and 6). Known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. Structure determination of pure compounds was accomplished primarily by extensive 1D- and 2D-NMR spectroscopy. The absolute configurations of compounds 1–5, 7 …
Hydroxyl Radical Is Produced Via The Fenton Reaction In Submitochondrial Particles Under Oxidative Stress: Implications For Diseases Associated With Iron Accumulation, Carin Thomas, Melissa M. Mackey, Amy A. Diaz, David P. Cox
Hydroxyl Radical Is Produced Via The Fenton Reaction In Submitochondrial Particles Under Oxidative Stress: Implications For Diseases Associated With Iron Accumulation, Carin Thomas, Melissa M. Mackey, Amy A. Diaz, David P. Cox
All Faculty Scholarship for the College of the Sciences
Mitochondrial dysfunction and reactive oxygen species (ROS) are often implicated in diseases involving oxidative stress and elevated iron. As mitochondria produce ATP by oxidative phosphorylation, ROS by-products are generated from the electron transport chain. Although superoxide and hydrogen peroxide have been thoroughly investigated, little evidence documents hydroxyl radical (HO•) production in mitochondria. In order to determine whether HO• is generated under oxidative stress conditions by a Fenton-type mechanism, bovine heart submitochondrial particles were examined for HO• in the presence and absence of iron ligands, antioxidant enzymes and HO• scavengers. HO• was measured as 2,3- and 2,5-dihydroxybenzoic acid (DHBA), using HPLC …
Sex- And Gonad-Affecting Scent Compounds And 3 Male Pheromones In The Rat, Jian-Xu Zhang, Lixing Sun, Jin-Hua Zhang, Zhi-Yong Feng
Sex- And Gonad-Affecting Scent Compounds And 3 Male Pheromones In The Rat, Jian-Xu Zhang, Lixing Sun, Jin-Hua Zhang, Zhi-Yong Feng
All Faculty Scholarship for the College of the Sciences
This study was aimed at identifying sex pheromones of the rat (Rattus norvegicus). We characterized the volatiles and semivolatiles of rat preputial gland and voided urine by using gas chromatography–mass spectrometry (GC–MS) and quantified them by their GC areas (abundances) and percentage of GC areas (relative abundances). Although all the compounds other than 4-heptanone and phenol detected were shared by males and females, the quantities for some of these sex-common compounds exhibited sexual dimorphism and decreased with gonadectomy. Thus, these compounds might be sex pheromones. Among them, squalene from preputial glands and 2-heptanone and 4-ethyl phenol from urine …
Spatial And Temporal Patterns In The Microbial Diversity Of A Meromictic Soda Lake In Washington State, Pedro A. Dimitriu, Holly C. Pinkart, Brent M. Peyton, Melanie R. Mormile
Spatial And Temporal Patterns In The Microbial Diversity Of A Meromictic Soda Lake In Washington State, Pedro A. Dimitriu, Holly C. Pinkart, Brent M. Peyton, Melanie R. Mormile
All Faculty Scholarship for the College of the Sciences
The microbial community diversity and composition of meromictic Soap Lake were studied using culture-dependent and culture-independent approaches. The water column and sediments were sampled monthly for a year. Denaturing gradient gel electrophoresis of bacterial and archaeal 16S rRNA genes showed an increase in diversity with depth for both groups. Late-summer samples harbored the highest prokaryotic diversity, and the bacteria exhibited less seasonal variability than the archaea. Most-probable-number assays targeting anaerobic microbial guilds were performed to compare summer and fall samples. In both seasons, the anoxic samples appeared to be dominated by lactate-oxidizing sulfate-reducing prokaryotes. High numbers of lactate- and acetate-oxidizing …
Land Use Influences The Spatiotemporal Controls On Nitrification And Denitrification In Headwater Streams, Clay P. Arango, J. L. Tank
Land Use Influences The Spatiotemporal Controls On Nitrification And Denitrification In Headwater Streams, Clay P. Arango, J. L. Tank
All Faculty Scholarship for the College of the Sciences
N and C cycles in headwater streams are coupled, and land use can modify these cycles by increasing N availability and removing riparian vegetation. To increase our understanding of how land use modifies the controls on N cycling, we quantified rates of 2 microbial N transformations in a total of 18 agricultural and urban streams (with and without riparian buffers) for 3 y to examine how riparian vegetation and land use influence sediment nitrification and denitrification. Nitrification rates were highest in agricultural streams in late spring. Nitrification was not related to streamwater NH4+ concentrations but was positively related …
Perchlorate And Nitrate Remediation Efficiency And Microbial Diversity In A Containerized Wetland Bioreactor, Paula Krauter, Bill Daily Jr., Valerie Dibley, Holly Pinkart, Tina Legler
Perchlorate And Nitrate Remediation Efficiency And Microbial Diversity In A Containerized Wetland Bioreactor, Paula Krauter, Bill Daily Jr., Valerie Dibley, Holly Pinkart, Tina Legler
All Faculty Scholarship for the College of the Sciences
We have developed a method to remove perchlorate (14–27 μg/L) and nitrate (48 mg/L) from contaminated groundwater using a wetland bioreactor. The bioreactor has operated continuously in a remote field location for more than 2 yr with a stable ecosystem of indigenous organisms. This study assesses the bioreactor for long-term perchlorate and nitrate remediation by evaluating influent and effluent groundwater for oxidation-reduction conditions and nitrate and perchlorate concentrations. Total community DNA was extracted and purified from 10-g sediment samples retrieved from vertical coring of the bioreactor during winter. Analysis by denaturing gradient gel electrophoresis of short, 16S rDNA, polymerase-chain-reaction products …
Photoreductive Dissolution Of Ferrihydrite By Methanesulfinic Acid: Evidence Of A Direct Link Between Dimethylsulfide And Iron-Bioavailability, Anne M. Johansen, Jennifer M. Key
Photoreductive Dissolution Of Ferrihydrite By Methanesulfinic Acid: Evidence Of A Direct Link Between Dimethylsulfide And Iron-Bioavailability, Anne M. Johansen, Jennifer M. Key
All Faculty Scholarship for the College of the Sciences
Within open‐ocean regions where excess macronutrients are present, phytoplankton growth is limited by the bioavailability of iron supplied to these areas primarily within atmospheric aerosols of crustal origin. However, processes that control the abundance of biologically accessible iron in these aerosols are largely unknown. Here we show that dissolution of ferrihydrite, a surrogate iron(oxy)hydroxide phase found in atmospheric waters, is enhanced in the presence of methanesulfinic acid (MSIA, CH3SO2H, a dimethylsulfide (DMS) oxidation intermediate) in laboratory irradiation experiments with aqueous suspensions that simulate marine aerosol particles. The increased release of soluble Fe(II) is attributed to a …
Aerobic Biodegradation Of Methyl Tert-Butyl Ether By Aquifer Bacteria From Leaking Underground Storage Tank Sites, S. R. Kane, H. R. Beller, T. C. Legler, C. J. Koester, Holly C. Pinkart, R. U. Halden, A. M. Happel
Aerobic Biodegradation Of Methyl Tert-Butyl Ether By Aquifer Bacteria From Leaking Underground Storage Tank Sites, S. R. Kane, H. R. Beller, T. C. Legler, C. J. Koester, Holly C. Pinkart, R. U. Halden, A. M. Happel
All Faculty Scholarship for the College of the Sciences
The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded in 15 days after a 4-day lag period), whereas no consumption of MTBE was observed for sediments from the other sites after 75 days. For sediments in which MTBE was consumed, 43 to 54% of added [U-14C]MTBE was mineralized to14CO2. Molecular phylogenetic analyses of these sediments indicated the enrichment of species closely …
Histochemical Investigation On The Distribution And Localization Of Digestive Enzymes In The Alimentary Canal Of The Caddis Fly Larvae, Brachycentrus Occidentalis Banks, Walter B. Hollow
All Master's Theses
A histochemical study of the digestive enzymes of Brachycentrus occidentalis Banks showed that the insect contains the enzymes necessary to utilize the major foods, viz., carbohydrates, lipids and proteins. There appears to be a correlation between the types of digestive enzymes found and the type of food eaten by the larvae. Values of pH for extracts from the different parts of the digestive system were: foregut, 7.6-8.4, midgut, 7.6-8.4, and hindgut, 7.0-7.6. Living micro-organisms in the gut lumen was also shown.
The Enzymatic Oxidation Of Aldehydes By Xanthine Oxidase, Duane Beal Gregory
The Enzymatic Oxidation Of Aldehydes By Xanthine Oxidase, Duane Beal Gregory
All Master's Theses
As early as sixty years ago, the existence of an enzyme from mammalian tissues which catalyzed the oxidation of xanthine and hypoxanthine was recognized. The name given the enzyme was derived from its earliest recognized action, hence xanthine oxidase. Although little is known about the biological functions of the enzyme, the high affinity shown for xanthine and hypoxanthine may indicate that these are the most significant substrates biologically.
Pigments And Neurogenic Secretions Of Dermasterias Imbricata, Stephen Edward Davis
Pigments And Neurogenic Secretions Of Dermasterias Imbricata, Stephen Edward Davis
All Master's Theses
Identification of the compound from the asteroid Dermasterias imbricata that evokes the swimming response in the sea anemone Stomphia coccinea was attempted. Although the compound was not identified, it was postulated to be a low molecular weight highly polar molecule of possible carbohydrate origin.
Purification And Properties Of Triosephosphate Isomerase From Selected Chlorophyta, John C. Meeks
Purification And Properties Of Triosephosphate Isomerase From Selected Chlorophyta, John C. Meeks
All Master's Theses
It is the purpose of this study to purify and investigate some of the properties of triosephosphate isomerase from two unicellular green algae 1 Ankistrodesmus Braunii and Scenedesmus acuminatus, and one filamentous green alga, Chara sp.
Pigments Of The Crinoid Florometra Serretissima, Guy Allen Howard
Pigments Of The Crinoid Florometra Serretissima, Guy Allen Howard
All Master's Theses
The purpose of the present study is to separate and identify the pigments responsible for the bright orange color of the crinoid Florometra serretissima. This is the first reported investigation of the pigmentation of this particular crinoid.