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Full-Text Articles in Life Sciences
The Effect Of Ammonia On The Germination And Outgrowth Of Bacillius Globigii, Joseph B. Kunicki
The Effect Of Ammonia On The Germination And Outgrowth Of Bacillius Globigii, Joseph B. Kunicki
Theses and Dissertations
This research investigated the germination and outgrowth of Bacillus globigii in the presence of ammonia in laboratory scale experiments. Germination was measured by monitoring the release of dipicolinic acid, an organic compound present in bacterial spores, while outgrowth was measured using phase-bright microscopy and semi-automated counting procedures. The ammonia-N concentrations investigated generally did not cause statistically significant differences in the initial 1-hr germination rates or the average 3-hr outgrowth rates of Bacillus globigii spores in batch style experiments. The average 1-hr germination rates observed in the absence of ammonia-N was 0.0258 hr-1 for the water and buffer controls and …
Production Of Recombinant Injectosome And Outer Membrane Proteins From Yersinia Pestis Kim5, Jeremiah N. Betz
Production Of Recombinant Injectosome And Outer Membrane Proteins From Yersinia Pestis Kim5, Jeremiah N. Betz
Theses and Dissertations
Molecular biology techniques and low cost reagents have lowered the barriers to entry for development of biological arsenals by non-state and state groups. Additionally, genetic engineering of epitope targets from such pathogens as Y. pestis would annul current detection methods and therapeutic treatments. Vaccines often have short shelf lives and are of minimal utility if not used prior to exposure to BW agents. Camelidae, including camels and llamas produce unique antibodies termed Nanobodies® (Nbs) or antigen specific fragments (VHH) which are much smaller than traditional antibodies (15 vs. ~150 kDal) yet seem to attach with the same …
Cell Modeling, Matthew L. Campbell
Cell Modeling, Matthew L. Campbell
Theses and Dissertations
The Air Force is currently developing new products that incorporate a variety of chemicals which may come in contact with product users. To define which chemicals are dangerous to the user, toxicity studies have been performed. However, analysis of toxicity ultimately requires models of the exposed cellular systems. This thesis provides an introduction of how to model and analyze small and large cellular systems. Understanding the underlying behavior of small models and their relation to large systems will lead to a better understanding of how the Air Force should construct intracellular models to assist in future toxicology studies. Developing analysis …