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Four Hapten Spacer Sites Modulating Class Specificity: Nondirectional Multianalyte Immunoassay For 31 Β-Agonists And Analogues., Lanteng Wang, Wenmeng Jiang, Xing Shen, Xiangmei Li, Xin-An Huang, Zhenlin Xu, Yuanming Sun, Shun-Wan Chan, Lingwen Zeng, Eremin Sergei Alexandrovich, Hongtao Lei
Four Hapten Spacer Sites Modulating Class Specificity: Nondirectional Multianalyte Immunoassay For 31 Β-Agonists And Analogues., Lanteng Wang, Wenmeng Jiang, Xing Shen, Xiangmei Li, Xin-An Huang, Zhenlin Xu, Yuanming Sun, Shun-Wan Chan, Lingwen Zeng, Eremin Sergei Alexandrovich, Hongtao Lei
Faculty of Science & Technology (THEi)
Immunoassay methods are important for monitoring β-agonists illegally used for reducing animal fat deposition in livestock. However, there is no simultaneous screening surveillance immunoassay for detecting various β-agonist chemicals that are possibly present in food. In this study, through the use of an R-(−)-salbutamol derivative as the immunizing hapten, an antibody recognizing 31 β-agonists and analogues was generated for the first time. Three-dimensional quantitative structure–activity relationship (3D QSAR) revealed that strong steric and hydrophobic fields around the hapten spacer near C-2, as well as a chirality at C-1′, dominantly modulated the class specificity of the raised antibody. However, a hapten …