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Dihydrosterculate In Tobacco Transformed With Bacterial Cyclopropane Fatty Acid Synthase, Katherine Schmid
Dihydrosterculate In Tobacco Transformed With Bacterial Cyclopropane Fatty Acid Synthase, Katherine Schmid
Katherine Schmid
Many gram negative bacteria accumulate cyclopropane fatty acids (CPFAs) in their membranes during stationary phase. In plants, on the other hand, CPFAs are best known as constituents of certain seed oils. CPFAs make up around 40% of Litchi chinensis seed oil [1] and a significant proportion of oils from other Sapindales. In order Malvales, small amounts of CPFA typically accompany cyclopropene fatty acids in seed oils, although up to 5% CPFA has been observed in the polar lipid fraction from Malvaceous roots [2]. Genetic engineering of oilseeds for unusual fatty acid production requires that the introduced fatty acids accumulate in …
Effects Of Cyclopropenoid Fatty Acids On Fungal Growth And Lipid Composition, Katherine Schmid, Glenn Patterson
Effects Of Cyclopropenoid Fatty Acids On Fungal Growth And Lipid Composition, Katherine Schmid, Glenn Patterson
Katherine Schmid
Cyclopropenoid fatty acids (CPE) isolated fromSterculia foetida oil by urea clathration and reverse phase high performance liquid chromatography (HPLC) were introduced into fungal cultures. Stearate levels in phospholipids and triacylglycerols fromUstilago maydis sporidia rose considerably in response to 30 μM CPE. In addition, CPE themselves were incorporated into glycerolipid fractions. Sterol composition was unaffected. Changes in lipid composition were accompanied by inhibition of dry weight accumulation and sporidial number. Treated sporidia showed irregular wall deposition and a branched morphology. Oleate alleviated CPE effects on growth and morphology. Hyphal extension byRhizoctonia solani was inhibited somewhat by 30 μM sterculate, whileFusarium oxysporum …