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Full-Text Articles in Life Sciences
Concanavalin A-Binding Enzymes Of Crotalus Scutulatus Scutulatus Venom, Michael W. Hinson, C. K. Childs, Bob D. Johnson, Dewey H. Sifford
Concanavalin A-Binding Enzymes Of Crotalus Scutulatus Scutulatus Venom, Michael W. Hinson, C. K. Childs, Bob D. Johnson, Dewey H. Sifford
Journal of the Arkansas Academy of Science
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The proteins binding to Con A exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC 3.1.3.2), phosphodiesterase, 5'-nucleotidase (5'-ribonucleotide phosphohydrolase EC 3.1.3.5), phospholipase A(phosphatidate 2-acylhydrolase EC 3.1.1 .4), hyaluronidase (hyaluronate glycanohydrolase EC 3.2.1 d), N-benzoyl-L-arginine ethyl esterase, p-toluenesulfonyl-L-arginine methyl esterase, L-amino acid oxidase (L-amino acid: 02 oxidoreductase [deaminating] EC 1.4.3.2), and caseinolytic activities. Thrombin-like and NAD nucleosidase (5'-ribonucleotide phosphohydrolase EC 3.1.3.5) activities were not observed. The crude venom and the fraction containing the glycoproteins which bound to Con A were fractionated by DEAE Sephadex A-50 ion …
Demonstration Of A Heat-Stable Cyclic Gmp Phosphodiesterase In The Medium Of Physarum Flavicomum, Thomas J. Lynch, Mark S. Ross
Demonstration Of A Heat-Stable Cyclic Gmp Phosphodiesterase In The Medium Of Physarum Flavicomum, Thomas J. Lynch, Mark S. Ross
Journal of the Arkansas Academy of Science
No abstract provided.
Effects Of Growth Temperatures On The Fatty Acid Composition Of Isolated Chloroplasts From Two Species Differing In Heat Sensitivity, Roy Z. Gehring, Glenn W. Todd
Effects Of Growth Temperatures On The Fatty Acid Composition Of Isolated Chloroplasts From Two Species Differing In Heat Sensitivity, Roy Z. Gehring, Glenn W. Todd
Journal of the Arkansas Academy of Science
Lipid analyses of chloroplasts isolated from wheat (Triticum aestivum L. cv. Arthur) and milo (Sorghum bicolor cv. Funk's hybrid 522) suggest no major heat effect on lipid class distribution. Assuming milo is more heat tolerant than wheat and that increased saturated/unsaturated fatty acid values increase thermal stability, changes in sulfoquinovosyldiglyceride (SL) appear to be more important than phosphatidylglycerol (PG) in conferring thermal stability to isolated chloroplasts.
Methemoglobin Reduction In The Presence Of Various Concentrations Of Oxyhemoglobin, Ali Mansouri
Methemoglobin Reduction In The Presence Of Various Concentrations Of Oxyhemoglobin, Ali Mansouri
Journal of the Arkansas Academy of Science
Methemoglobin reduction in human red blood cell is governed by many factors, one of which is the concentration of oxyhemoglobin. Experiments were conducted to determine the inhibition of methemoglobin reduction by oxyhemoglobin under concentrations approaching those in the red cell. Hemoglobin, methemoglobin reductase and cytochrome b₅ were prepared by routine protein purification methods. Methemoglobin reduction was carried out at 37° C in spectrophotometer. It is shown that methemoglobin concentration variation (at least when it is above 1 mM) does not change the reduction rate. Whereas, the ratio of methemoglobin to oxyhemoglobin determines the rate of methemoglobin reduction when all other …