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Full-Text Articles in Life Sciences
Characterization Of A Split-Nluc Assay For Monitoring Changes In Alpha-Synuclein Solubility In Living Cells, Baolong Truong
Characterization Of A Split-Nluc Assay For Monitoring Changes In Alpha-Synuclein Solubility In Living Cells, Baolong Truong
Honors Theses
Alpha-synuclein is the primary constituent of Lewy Bodies, protein aggregates associated with Parkinson’s Disease, Dementia with Lewy Bodies, and Multiple Systems Dystrophy. Given the terminal nature of these synucleinopathies, the need for an assay that can report on alpha-synuclein aggregation levels in living systems is critical. Western blotting represents one potential approach to this problem; however, this technique can often be limited by selectivity of available antibodies and experimental reproducibility. Herein, we describe a novel luminescence-based assay capable of detecting alpha-synuclein solubility in bacterial cells. This platform can report on the influence of disease-relevant mutations and small molecules. Antibodies are …
Development And Implementation Of A Biochemistry Argumentation Task To Promote Scientific Literacy Among Undergraduate Students, Lukas Hall
Honors Theses
Science education reform efforts at the postsecondary level have been lacking regardless of meaningful reform at the K-12 level. Of these reforms, the Next Generation Science Standards (NGSS) serve to reframe effective science teaching and learning as three-dimensional (3D). That is, 3D instruction integrates core disciplinary ideas, cross-cutting concepts, and science practices to support students’ science learning. There have been calls to extend this 3D model to the postsecondary level. The purpose of this study was to design, implement, and evaluate a 3D task in a university-level biochemistry course. The task was implemented as a homework assignment to 107 students …