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Full-Text Articles in Life Sciences
Thermodynamics And Conformational Heterogeneity Of Recbcd Binding To Dna Ends, Linxuan Hao
Thermodynamics And Conformational Heterogeneity Of Recbcd Binding To Dna Ends, Linxuan Hao
Arts & Sciences Electronic Theses and Dissertations
E. coli RecBCD is crucial in initiating repair of double stranded (ds) DNA breaks. It is a heterotrimeric helicase and nuclease complex possessing two ATPase motors, RecB and RecD, and a regulatory subunit without ATPase activity, RecC. The RecB subunit also contains a 30kDa nuclease domain (RecBNuc) that, according to published structural data, is situated over 60Å away from the site of dsDNA binding. Surprisingly, we have shown in previous studies that deletion of RecBNuc to form RecBΔNucCD affects its dsDNA unwinding properties. The mechanism by which RecBNuc influences RecBCD dsDNA unwinding is unclear. In this thesis, equilibrium binding techniques, …
Structural Mechanism Of Poxvirus Sabotage Of T-Cell Costimulation, Jabari Issa Elliott
Structural Mechanism Of Poxvirus Sabotage Of T-Cell Costimulation, Jabari Issa Elliott
Arts & Sciences Electronic Theses and Dissertations
Poxviruses are characterized by large double stranded DNA genomes that encode numerous proteins tailored for host immune response evasion. Our lab has been investigating a sequence-diverse family of secreted poxvirus proteins that appear to share a conserved beta-sandwich fold, but differ in their immunomodulatory functions. We have termed members of this superfamily Poxvirus Immune Evasion (PIE) proteins, and there appears to be at least 20 distinct subfamilies. As it turns out, cowpox virus (CPXV) encodes 10 PIE proteins, one of which, M2, can inhibit murine T cell activation through specific interactions with co-stimulatory ligands B7. 1 (CD80) and B7. 2 …