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Structural And Biochemical Analyses Of Dna And Rna Binding By A Bifunctional Homing Endonuclease And Group I Intron Splicing Factor, Jill M. Bolduc, P. Clint Spiegel, Pivali Chatterjee, Kristina L. Brady, Maureen E. Downing, Mark G. Caprara, Richard B. Waring, Barry L. Stoddard

Chemistry Faculty and Staff Publications

We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 Å resolution. The structure demonstrates the remarkable structural conservation of the (3-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant …