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Blood Coagulation Factor Ix: Purification, Activation, Crystallization, Juliet Mcgill
Blood Coagulation Factor Ix: Purification, Activation, Crystallization, Juliet Mcgill
WWU Honors College Senior Projects
This paper presents readers with an optimized procedure for the purification, activation, and crystallization of selected blood coagulation Factor IX double mutant (FIX_2). Through the completion of this work, we aim to enhance future biochemical and structural studies by providing an easier means for the FIX_2 production, in order to increase understanding of the protein’s function within the blood coagulation cascade. The initiation of the blood coagulation cascade is brought on by activation of inactive Factor VIII (FVIII) protein though contact with tissue factor, the FVIII protein then binds to an activated platelet surface where it must wait for its …
Blood Coagulation Factor Ix: Purification, Isolation, Activation, Alex Macneil
Blood Coagulation Factor Ix: Purification, Isolation, Activation, Alex Macneil
WWU Honors College Senior Projects
This paper attempts to provide an optimized strategy for the purification, activation, and isolation of blood coagulation Factor IX mutants. The goal of this work is to enable future biochemical and structural studies of Factor IX to a gain a better understanding of the structural-functional role this protein plays in the blood coagulation cascade. The orchestration and amplification of the blood coagulation cascade requires the binding of Factor VIII (FVIII) to an activated platelet surface, where it serves as a cofactor to a serine protease, Factor IX (FIX). Factor IX circulates the bloodstream as a catalytically silent multidomain protein1. Like …