Life Sciences Commons^™

The Flavoprotein Rcla Is A Hypothiocyanous Acid Reductase, Irina Chapman

Masters Theses

Reactive chlorine species (RCS) - hypochlorous acid (HOCl), hypothiocyanous acid (HOSCN), and chloramines - are powerful antimicrobial oxidants generated by the innate immune system. Bacteria developed defense mechanisms against RCS, which are highly toxic for invading pathogens. One such defense system involves RclA, an enzyme from the flavin disulfide reductase family, which is massively upregulated upon exposure of Escherichia coli to RCS. Recent research suggests that RclA functions as a Cu(II) reductase to provide resistance against RCS. However, the exact mechanism used by RclA was subject to debate. In this study, we demonstrate that RclA is unlikely to function as …

Expression Of Insulin Responsive Genes In Insulin Resistant Conditions, And The Effect Of Selenium On Gene Expression, David L. Ruff

Masters Theses

Chronically high blood glucose levels lead to many problems, such as insulin resistance, the hallmark of Type II diabetes. Increased flux through the hexosamine biosynthesis pathway is one mechanism by which high glucose as well as glucosamine has been shown to induce insulin resistance. This study tests the effects of glucosamine induced insulin resistance on insulin regulation of the metabolic genes glucose-6-phosphate dehydrogenase (G6PDH) and fatty acid synthase (FAS) as well as insulin responsive proteins tribbles homolog (TRIB3) and sterol regulatory element binding protein (SERBP-1c) 1c.

Selenium, a micronutrient has been shown to be an effective insulin mimetic in Type …

Isolation And Characterization Of The Triiodothyronine Regulatory Region Of The Fatty Acid Synthase Gene In Avian Species, Delfina Rivera Valencia

Masters Theses

Fatty acids are utilized for energy storage in the form of triglycerides or neutral fats. One way to regulate fatty acid biosynthesis is to regulate the activity of fatty acid synthase (FAS), the enzyme that catalyzes the reaction. Previous studies have shown that regulation of FAS activity and synthesis is regulated by the hormone triiodothyronine, T₃, at the level of transcription. Thus, isolation and characterization of the promoter region o f the FAS gene will enable the identification of the thyroid hormone response elements (TREs). This study could shed light on the mechanisms on how this gene can …

The Effects Of Selenate, Molybdate And Chromate On The Triiodothyronine Induced Enzyme Activity And Mrna Level For Both Fatty Acid Synthase And Malic Enzyme, Yuan Zhu

Masters Theses

In chick embryo hepatocytes in culture, triiodothyronine (T3) stimulates enzyme activity, mRNA level and transcription rate for both fatty acid synthase (FAS) and malic enzyme (ME). Recent evidence has demonstrated the effects of selenate, molybdate and chromate on various physiological processes. Little information, however, is available on the effects of these metal ions on the T3- regulated gene expression for FAS and ME.

In chick embryo hepatocytes incubated in a chemically defined medium, addition of sodium selenate (20 μM) coincident with T3 almost completely inhibited the T3-induced stimulation of FAS and ME activity and accumulation of their respective mRNA's. Neither …

Intracellular Protein Degradation In A Mesophile And A Thermophile Of The Genus Bacillus, Cameron C. Hoover

Masters Theses

¹⁴C-leucine was incorporated into th e proteins of the mesophile Bacillus licheniformis and the thermophile Bacillus stearothermophilus. Aliquots of the cell suspension were treated with chloramine-T in order to decarboxylate the ¹⁴C-leucine that was released as the proteins degraded. The ¹⁴CO₂ was trapped and counted. This allowed for the determination of the extent of intracellular protein degradation at various tem peratures and at various stages of the growth cun/e. For the mesophile, the extent of degradation increased with temperature from 30° C to 55° C. For the thermophile, no significant amount of degradation occurred until …

Fractionation Of Ribosomal Proteins By Affinity Chromatography, Corazon E. Victoria

Masters Theses

No abstract provided.

Comparison Of Protein Kinase Activity In Tissue Extracts Of The Diabetic And Nondiabetic Chinese Hamster, Michael Andrew Dombos

Masters Theses

No abstract provided.

The Half-Life Of Bacterial Messenger Rna, John B. Madison

Masters Theses

No abstract provided.

Fractionation Of Ribosomal Proteins From Mesophilic And Thermophilic Bacteria, Raymond J. Knaus

Masters Theses

No abstract provided.

The Isolation And Partial Structure Identification Of A Polar Glycosphingolipid Of Rabbit Blood, Michael B. Shubeck

Masters Theses

No abstract provided.

Two-Dimensional Gel Electrophoresis Of Ribosomal Proteins From Thermophilic And Mesophilic Bacteria, Walter C. Pickett

Masters Theses

No abstract provided.

Binding Of Aminoacyl-Trna To Bacterial Ribosomes, Somesh Datt Sharma

Masters Theses

No abstract provided.

Fraction Of Bacterial Ribosomes, Katty S. F. H. Yang

Masters Theses

No abstract provided.

Triplet-Triplet Energy Transfer In Enzyme-Inhibitor Complexes, Ronald Earl Hauxwell

Masters Theses

No abstract provided.

A Comparison Of Luteinizing Hormone And Prostaglandin F2 Α Stimulation Of Pseudo-Pregnant Rat Ovarian Steroidogenesis In Vitro, Ross R. Erickson

Masters Theses

No abstract provided.

Characterization Of Some Unidentified Components In Aorta Lipids, Max Elvin Royer

Masters Theses

No abstract provided.

Membrane Bound Dehydrogenase Enzymes And Related Electron Transport Systems In Acetobacter Aceti Var. Liquefaciens: Their Induction And Pleiotropic Nature, John C. Rakoczy

Masters Theses

No abstract provided.

The Effect Of Puromycin On Protein Synthesis Of Cell Free Systems From Mesophilic And Thermophilic Bacteria, Philip Y. Shen

Masters Theses

No abstract provided.

Concentration And Fatty Acid Distribution Of Aortic Cerebrosides In Cholesterol-Fed And Normal Rabbits, Robert V. P. Tao

Masters Theses

No abstract provided.