Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Test-Driving The Next Generation Of Crispr Gene Editing, Olivia Gornichec, Kailey Mayer
Test-Driving The Next Generation Of Crispr Gene Editing, Olivia Gornichec, Kailey Mayer
Research on Capitol Hill
USU team Kailey, recent graduate of animal science, and Olivia, senior in biochemistry, have led and funded this project through a student grant. CRISPR has been making waves in the scientific community for its potential to help us edit genomes. However, that is just one of the known types of CRISPR, and other types aren’t in forms that are accessible to study. Kailey and Olivia have successfully cloned Type IV-B into a plasmid that can now be used to perform further research into what this system does. The two students never expected that, as undergrads, they would make a foundation-level …
Molecular Relatedness Of Two Distinct Type Iv Crispr-Associated (Cas) Proteins, Matt Armbrust
Molecular Relatedness Of Two Distinct Type Iv Crispr-Associated (Cas) Proteins, Matt Armbrust
Research on Capitol Hill
CRISPR-Cas systems are prokaryotic adaptive immune systems. Bacteria use CRISPR systems as a defense against foreign nucleic acid invasion such as phage infection.
Role Of Nucleotide Identity In Effective Crispr Target Escape Mutations, Tim Künne, Yifan Zhu, Fausia Da Silva, Nico Konstantinides, Rebecca E. Mckenzie, Ryan N. Jackson, Stan J.J. Brouns
Role Of Nucleotide Identity In Effective Crispr Target Escape Mutations, Tim Künne, Yifan Zhu, Fausia Da Silva, Nico Konstantinides, Rebecca E. Mckenzie, Ryan N. Jackson, Stan J.J. Brouns
Chemistry and Biochemistry Faculty Publications
Prokaryotes use primed CRISPR adaptation to update their memory bank of spacers against invading genetic elements that have escaped CRISPR interference through mutations in their protospacer target site. We previously observed a trend that nucleotide-dependent mismatches between crRNA and the protospacer strongly influence the efficiency of primed CRISPR adaptation. Here we show that guanine-substitutions in the target strand of the protospacer are highly detrimental to CRISPR interference and interference-dependent priming, while cytosine-substitutions are more readily tolerated. Furthermore, we show that this effect is based on strongly decreased binding affinity of the effector complex Cascade for guanine-mismatched targets, while cytosine-mismatched targets …