Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
An Enzyme-Linked Immunosorbent Assay (Elisa) For Pantothenate, Allen H. Smith
An Enzyme-Linked Immunosorbent Assay (Elisa) For Pantothenate, Allen H. Smith
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
An enzyme-linked immunosorbent assay (ELISA) for pantothenate has been developed. Antibodies induced in rabbits against bovine serum albumin-pantothenate conjugate were specifically purified by affinity chromatography. This process served to reduce the amount of endogenous pantothenate attached to the antibody, as well as to purify the antibody. The purified antibodies were covalently linked to alkaline phosphatase (Sigma type VII) with glutaraldehyde (0.05% aqueous solution). An immobilized pantothenate substrate was first obtained by attaching human serum albumin-pantothenate conjugate to the surface of polystyrene culture tubes by passive adsorption. The binding of the enzyme labelled antibody (E-AB) to this substrate is proportionately inhibited …