Life Sciences Commons^™

Identification Of Soybean Lipoxygenase Products By Gas Chromatography-Mass Spectrometry (Ge-Ms), Rehan Ahmad Malik

Journal of Undergraduate Research at Minnesota State University, Mankato

Lipoxygenases (LOXs) are enzymes which catalyze peroxidation of polyunsaturated fatty acids containing at least one cis, cis 1, 4-pentadiene moiety to form conjugated diene-hydroperoxides. Soybean seeds contain three LOX isoenzymes while at least five different isoenzymes are in the vegetative tissue. Lipoxygenases have been related to several functions i.e. plant growth, defense mechanisms against pathogens and pests, and lipid metabolism. Given the proposed roles of LOX isoenzymes and the presence of multiple LOX isoenzymes in soybean vegetative tissue, it is likely that individual isoenzymes play specific functions. Do the isoenzymes show differences in which products they form? The basic goal …